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Recombinant Mouse Ahr protein, His/GST-tagged

Cat.No. : Ahr-145M
Product Overview : Recombinant Mouse Ahr(Val126~Gly385) fused with His/GST tag at N-terminal was expressed in E. coli.
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Description : The protein encoded by this gene is a ligand-activated helix-loop-helix transcription factor involved in the regulation of biological responses to planar aromatic hydrocarbons. This receptor has been shown to regulate xenobiotic-metabolizing enzymes such as cytochrome P450. Before ligand binding, the encoded protein is sequestered in the cytoplasm; upon ligand binding, this protein moves to the nucleus and stimulates transcription of target genes. Two transcript variants encoding different isoforms have been found for this gene.
Source : E. coli
Species : Mouse
Tag : His/GST
Form : 20mM Tris, 150mM NaCl,(pH8.0,1mM EDTA, 1mM DTT, 0.01%skl, 5% Trehalose and Proclin300)
Molecular Mass : 59.5kDa
Protein length : Val126~Gly385
Endotoxin : <1.0EU per 1µg (determined by the LAL method)
Purity : > 95%
Applications : Positive Control; Immunogen; SDS-PAGE; WB.
If bio-activity of the protein is needed, please check active protein
Stability : The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Storage : Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
Reconstitution : Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Gene Name : Ahr aryl-hydrocarbon receptor [ Mus musculus ]
Official Symbol : Ahr
Synonyms : AHR; aryl-hydrocarbon receptor; aryl hydrocarbon receptor; ah receptor; dioxin receptor; Ah; In; Ahh; Ahre; bHLHe76;
Gene ID : 11622
mRNA Refseq : NM_013464
Protein Refseq : NP_038492
UniProt ID : P30561

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (10)

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What are the genetic and epigenetic factors that regulate AHR expression and function? 12/19/2022

The expression and function of AHR can be influenced by genetic polymorphisms, DNA methylation patterns, and histone modifications, which may have implications in individual susceptibility to environmental toxins.

How is AHR involved in skin biology and the response to environmental toxins? 09/28/2022

AHR is expressed in skin cells and plays a role in the response to environmental toxins, UV radiation, and the regulation of skin barrier function.

What is the role of AHR in embryonic development and organogenesis? 06/07/2022

AHR is essential for normal embryonic development and organogenesis. It participates in the patterning and differentiation of various organs, including the heart and vasculature.

Can AHR activation affect hormonal signaling pathways? 03/15/2022

Yes, AHR activation can interfere with hormonal signaling pathways, leading to cross-talk with estrogen receptor signaling, and affecting hormonal homeostasis.

How does AHR influence cellular proliferation and apoptosis? 12/06/2021

AHR can impact cell cycle progression and apoptosis by modulating the expression of genes involved in these processes, which can have implications in cancer biology.

What role does AHR play in the immune system and inflammation? 09/09/2021

AHR is involved in the regulation of immune responses and inflammation by affecting the differentiation and function of immune cells, such as T cells and dendritic cells.

How does AHR contribute to xenobiotic metabolism and detoxification? 06/25/2021

AHR activation induces the expression of cytochrome P450 enzymes, particularly CYP1A1 and CYP1B1, which metabolize and detoxify various environmental pollutants and xenobiotics.

What are the downstream targets of AHR signaling, and how is gene expression regulated by AHR? 03/04/2021

AHR forms a complex with co-activators or co-repressors upon ligand binding, leading to the modulation of gene expression. Downstream targets include cytochrome P450 enzymes (CYP1A1, CYP1B1), phase II detoxification enzymes, and cytokines.

How does AHR activation occur, and what are its endogenous ligands? 12/13/2020

AHR activation occurs upon binding to specific ligands, including polycyclic aromatic hydrocarbons (PAHs), halogenated aromatic hydrocarbons (HAHs), and tryptophan derivatives, such as indole-3-carbinol. Endogenous ligands are derived from cellular metabolism and the diet.

How is AHR activity regulated post-translationally, and what are the protein-protein interactions that modulate its function? 04/18/2018

AHR activity can be regulated through various post-translational modifications, including phosphorylation and ubiquitination. Protein-protein interactions with chaperones and co-regulators also influence AHR function.

Customer Reviews (5)

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Reviews
09/01/2022

    Thumbs up for this lab protein – it's top-notch!

    02/13/2022

      I'm loving this lab protein – it's a research game-changer.

      07/14/2021

        Five stars for this incredible lab protein – highly recommend.

        08/07/2018

          The lab protein's performance has exceeded our expectations, and its versatility has enabled diverse applications within our research scope.

          06/13/2018

            We've seen remarkable results with this lab protein.

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