Recombinant Mouse Atp5b Protein, MYC/DDK-tagged


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Recombinant Mouse Atp5b Protein, MYC/DDK-tagged

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Cat.No. : Atp5b-669M
Product Overview : Purified recombinant protein of full-length mouse ATP synthase, H+ transporting mitochondrial F1 complex, beta subunit (Atp5b), with C-terminal MYC/DDK tag, expressed in HEK293T cells.
Description : Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F(1). Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits.
Source : HEK293T
Species : Mouse
Molecular Mass : 56.3 kDa
Purity : >80% as determined by SDS-PAGE and Coomassie blue staining
Stability : Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Storage : Store at -80 centigrade after receiving vials.
Concentration : >50 μg/mL as determined by microplate BCA method
Storage Buffer : 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.
Gene Name : Atp5b ATP synthase, H+ transporting mitochondrial F1 complex, beta subunit [ Mus musculus (house mouse) ]
Official Symbol : Atp5b
Synonyms : Atp5b; ATP synthase, H+ transporting mitochondrial F1 complex, beta subunit; Atp5f1b; ATP synthase subunit beta, mitochondrial; ATP synthase F1 subunit beta; ATP synthase, H+ transporting mitochondrial F1 complex, alpha subunit; mitochondrial ATP synthase, H+ transporting F1 complex beta subunit; EC
Gene ID : 11947
mRNA Refseq : NM_016774
Protein Refseq : NP_058054
UniProt ID : P56480
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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