|Product Overview :
||Recombinant mouse BACE2 (Q9JL18) (Met1-Pro462) was expressed with a C-terminal polyhistidine tag.
||BACE2, also known as beta secretase 2, belongs to the peptidase A1 family. It is a protease known to be an important enzyme involved in the cellular pathways. BACE2 has been shown to interact with GGA1 and GGA2. It is the major β-secretase in vivo. BACE2 is located on chromosome 21 and may play a role in alzheimer's disease pathogenesis in down syndrome(DS). Overexpression of BACE2 by lentivirus markedly reduced amyloid β protein production in primary neurons. Despite an extra copy of the BACE2 gene in DS and the increase of its transcription, BACE2 protein levels are unchanged.
||Sterile PBS, pH 7.4
||Measured by its ability to cleave a fluorogenic peptide substrate Mca-KPLGL-Dpa-AR-NH2.
The specific activity is >50 pmoles/min/μg.
|Molecular Mass :
||The recombinant mouse BACE2 comprises 454 amino acids and has a predicted molecular mass of 49.2 kDa. The apparent molecular mass of the protein is approximately 55 kDa in SDS-PAGE under reducing conditions due to glycosylation.
||< 1.0 EU per μg of the protein as determined by the LAL method
||> 95 % as determined by SDS-PAGE
||Store it under sterile conditions at -20 centigrade to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.