Active Recombinant Mouse Car14 Protein, His-tagged
Cat.No. : | Car14-822M |
Product Overview : | Recombinant extracellular domain of mouse Car14 (NP_035927.1) (Met 1-Met 290) was expressed with a C-terminal polyhistidine tag. |
- Specification
- Gene Information
- Related Products
Source : | HEK293 |
Species : | Mouse |
Tag : | His |
Predicted N Terminal : | Ala 16 |
Form : | Lyophilized from sterile 25mM Tris, 0.15mM NaCl, pH 7.51, 5%~8% trehalose and mannitol. |
Bio-activity : | Measured by its esterase activity. The specific activity is >400 pmoles/min/μg, as measured with 1 mM 4-Nitrophenyl acetate and 0.8 μg enzyme at 400 nm in 100 μL of 12.5 mM Tris, 75 mM NaCl, pH 7.5. |
Molecular Mass : | The recombinant mouse CA14 consists of 286 amino acids and has a calculated molecular mass of 32.2 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 45-48 kDa protein in SDS-PAGE under reducing conditions. |
Endotoxin : | < 1.0 EU per μg of the protein as determined by the LAL method. |
Purity : | >95 % as determined by SDS-PAGE. |
Stability : | Samples are stable for up to twelve months from date of receipt at -70ºC. |
Storage : | Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents. |
Gene Name : | Car14 carbonic anhydrase 14 [ Mus musculus ] |
Official Symbol : | Car14 |
Synonyms : | Ca14; AW536446; CA XIV; CA-XIV; carbonate dehydratase XIV; carbonic anhydrase XIV |
Gene ID : | 23831 |
mRNA Refseq : | NM_011797 |
Protein Refseq : | NP_035927 |
MIM : | |
UniProt ID : | Q9WVT6 |
Chromosome Location : | 3; 3 F2.1 |
Pathway : | Metabolism, organism-specific biosystem; Nitrogen metabolism, organism-specific biosystem; Nitrogen metabolism, conserved biosystem |
Function : | carbonate dehydratase activity; lyase activity; metal ion binding |
Products Types
◆ Recombinant Protein | ||
CA14-118H | Recombinant Human CA14 Protein, His-tagged | +Inquiry |
CA14-0236H | Recombinant Human CA14 Protein, GST-Tagged | +Inquiry |
CA14-1156M | Recombinant Mouse CA14 Protein, His (Fc)-Avi-tagged | +Inquiry |
Car14-1960M | Recombinant Mouse Car14 Protein, Myc/DDK-tagged | +Inquiry |
CA14-2030H | Recombinant Human CA14 Protein, MYC/DDK-tagged | +Inquiry |
◆ Lysates | ||
CA14-2802HCL | Recombinant Human CA14 cell lysate | +Inquiry |
CA14-3064MCL | Recombinant Mouse CA14 cell lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (7)
Ask a questionSpecific modulators can be identified through high-throughput screening assays targeting CA14 and validated using in vitro and in vivo models.
CA14's unique functional attributes can be discerned using comparative enzymatic assays and structural analyses against other isoforms.
Influence of pH or ions on CA14 can be analyzed using enzymatic assays under varying conditions and ion-sensitive probes.
Disease associations can be determined through genetic studies, patient tissue analyses, and literature reviews focusing on CA14's relevance in pathological conditions.
Predominant tissue or cell-type expression can be determined using tissue microarrays, qPCR, or proteomic databases, correlating findings with potential physiological implications.
Consequences of overexpression or knockdown can be assessed using phenotypic analyses, cellular viability assays, and in vivo studies in organisms with altered CA14 expression.
CA14's role in cellular pathways can be explored using pathway enrichment analyses, proteomic studies, and pathway-specific assays in CA14-modified cells.
Customer Reviews (3)
Write a reviewReliable source, ensures reproducibility in our research.
Efficient services, optimize our protein-related studies.
Responsive customer support, aids in problem-solving effectively.
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