Ccl12-621M
Optional Service: Optional requirements on this protein
Product Overview :
Recombinant Mouse Ccl12 protein was expressed in Escherichia coli.
Description :
Murine CCL12 is belonging to the CC chemokine family. It shows 66 % amino acid sequence identity to human MCP-1. CCL12 has been shown to be a potent chemoattractant for monocytes and lymphocytes but not neutrophils. At high concentrations, it will also chemoattract eosinophils. CCL12 can induce a calcium flux in human HEK-293 cells transfected with murine CCR2, but can not induce a calcium flux in HEK-293 cells transfected with CCR1, CCR3, or CCR5. That shows the functional receptor of CCL12 is CCR2.
Source :
E.coli
Species :
Mouse
Form :
Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH 7.4.
Bio-activity :
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood monocytes is in a concentration range of 10-50 ng/ml.
Molecular Mass :
Approximately 9.3 kDa, a single non-glycosylated polypeptide chain containing 82 amino acids.
Protein length :
82
AA Sequence :
GPDAVSTPVT CCYNVVKQKI HVRKLKSYRR ITSSQCPREA VIFRTILDKE ICADPKEKWV KNSINHLDKT SQTFILEPSC LG
Endotoxin :
Less than 1 EU/μg of rMuMCP-5/CCL12 as determined by LAL method.
Purity :
>95% by SDS-PAGE and HPLC analysis.
Storage :
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution :
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤-20 centigrade. Further dilutions should be made in appropriate buffered solutions.
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.