- Mouse(6)
Recombinant Mouse Ccl12 protein
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Cat.No. : | Ccl12-621M Optional Service: Optional requirements on this protein |
Product Overview : | Recombinant Mouse Ccl12 protein was expressed in Escherichia coli. |
Description : | Murine CCL12 is belonging to the CC chemokine family. It shows 66 % amino acid sequence identity to human MCP-1. CCL12 has been shown to be a potent chemoattractant for monocytes and lymphocytes but not neutrophils. At high concentrations, it will also chemoattract eosinophils. CCL12 can induce a calcium flux in human HEK-293 cells transfected with murine CCR2, but can not induce a calcium flux in HEK-293 cells transfected with CCR1, CCR3, or CCR5. That shows the functional receptor of CCL12 is CCR2. |
Source : | E.coli |
Species : | Mouse |
Form : | Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH 7.4. |
Bio-activity : | Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood monocytes is in a concentration range of 10-50 ng/ml. |
Molecular Mass : | Approximately 9.3 kDa, a single non-glycosylated polypeptide chain containing 82 amino acids. |
Protein length : | 82 |
AA Sequence : | GPDAVSTPVT CCYNVVKQKI HVRKLKSYRR ITSSQCPREA VIFRTILDKE ICADPKEKWV KNSINHLDKT SQTFILEPSC LG |
Endotoxin : | Less than 1 EU/μg of rMuMCP-5/CCL12 as determined by LAL method. |
Purity : | >95% by SDS-PAGE and HPLC analysis. |
Storage : | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, -20 to -70 centigrade under sterile conditions after reconstitution. |
Reconstitution : | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤-20 centigrade. Further dilutions should be made in appropriate buffered solutions. |
Gene Name : | Ccl12 |
Official Symbol : | Ccl12 |
Synonyms : | CCL12; chemokine (C-C motif) ligand 12; C-C motif chemokine 12; MCP-1-related chemokine; small inducible cytokine A12; small-inducible cytokine A12; monocyte chemotactic protein 5; monocyte chemoattractant protein 5; MCP-5; Scya12; |
Gene ID : | 20293 |
mRNA Refseq : | NM_011331 |
Protein Refseq : | NP_035461 |
UniProt ID : | Q62401 |
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CCL1CCL11Ccl12CCL13CCL14CCL15CCL16CCL17CCL18CCL19CCL2CCL20CCL21Ccl21cCCL22CCL23CCL24CCL25CCL26CCL27Ccl27aCCL28CCL3CCL3L1Ccl4CCL5Ccl6CCL7CCL8Ccl9CCL4L2Ccl21aCCL4L1CCL3L3Ccl21bCcl27b
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