Recombinant Mouse Cd86 Protein, His-tagged, FITC conjugated

Cat.No. : Cd86-2281MF
Product Overview : FITC conjugated recombinant Mouse Cd86 (Val24-Glu245) protein was fused to His-tag at C-terminus and expressed in human 293 cells (HEK293).
Availability July 19, 2024
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Description : Cluster of Differentiation 86 (CD86) is also known as B-lymphocyte activation antigen B7-2, is a type I membrane protein that is a member of the immunoglobulin superfamily, and is constitutively expressed on interdigitating dendritic cells, Langerhans cells, peripheral blood dendritic cells, memory B cells, and germinal center B cells. Additionally, B72 is expressed at low levels on monocytes and can be upregulated through interferon γ. CD86 is the ligand for two different proteins on the T cell surface: CD28 (for autoregulation and intercellular association) and CTLA-4 (for attenuation of regulation and cellular disassociation). CD86 works in tandem with CD80 to prime T cells. Recent study has revealed that B7-2 promotes the generation of a mature APC repertoire and promotes APC function and survival. Furthermore, the B7 proteins are also involved in innate immune responses by activating NF-κB-signaling pathway in macrophages. CD86 thus is regarded as a promising candidate for immune therapy. CD86+ macrophages in Hodgkin lymphoma patients are an independent marker for potential nonresponse to firstline-therapy.
Source : HEK293
Species : Mouse
Tag : His
Form : Lyophilized
Molecular Mass : The protein has a calculated MW of 27.1 kDa. The protein migrates as 40-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Protein length : Val24-Glu245
N-terminal Sequence Analysis : Val 24
Endotoxin : < 1.0 EU/ μg by the LAL method.
Purity : > 95 % as determined by SDS-PAGE
Characteristic : Disulfide-linked homodimer
Labeled with FITC via amines
Excitation source: 488 nm spectral line, argon-ion laser
Excitation Wavelength: 488 nm
Emission Wavelength: 535 nm
Storage : For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20 to -70 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
Storage Buffer : Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% trehalose.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 μg/μL. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.
Gene Name : Cd86
Official Symbol : Cd86
Synonyms : CD86; CD86 antigen; T-lymphocyte activation antigen CD86; activation B7-2 antigen; early T cell costimulatory molecule-1; early T-cell costimulatory molecule 1; B7; B70; MB7; B7-2; B7.2; CLS1; Ly58; ETC-1; Ly-58; MB7-2; Cd28l2; TS/A-2
Gene ID : 12524
mRNA Refseq : NM_019388
Protein Refseq : NP_062261
UniProt ID : P42082

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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01/09/2022

    With the CD86 protein's high-quality composition and reliability, researchers can confidently proceed with their studies, knowing that they have access to a protein that consistently delivers accurate results.

    05/30/2021

      Its high purity and stability ensure reliable and reproducible results, which are paramount for achieving meaningful scientific outcomes.

      12/08/2017

        The CD86 protein is renowned for its outstanding quality, making it an optimal choice to fulfill the requirements of my experimental investigations.

        Q&As (5)

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        Are there any ongoing clinical trials involving CD86 inhibitors? 02/18/2023

        Yes, several clinical trials are investigating the efficacy of CD86 inhibitors in various diseases, including autoimmune disorders and certain cancers.

        How does CD86 contribute to transplant medicine? 02/03/2021

        In transplantation, CD86 blockade is investigated as a potential strategy to modulate the immune response and prevent graft rejection by inhibiting excessive T cell activation.

        How does CD86 influence the balance between Th1 and Th2 immune responses? 08/26/2019

        CD86 signaling is involved in the differentiation of T helper cells. Modulating CD86 levels may influence the balance between Th1 and Th2 responses, which is relevant in various immune-mediated diseases.

        Can CD86 be used as a prognostic marker in certain diseases? 12/29/2018

        There is emerging evidence suggesting that CD86 expression levels may have prognostic value in certain diseases, guiding clinicians in predicting disease outcomes.

        How does CD86 influence the development of regulatory T cells (Tregs)? 10/02/2016

        CD86 signaling is involved in the development and function of regulatory T cells, which play a crucial role in maintaining immune tolerance and preventing autoimmune reactions.

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