Recombinant Mouse Cdc20 Protein, MYC/DDK-tagged

• Cat.No.: Cdc20-352M
• Product Overview: Purified recombinant protein of full-length mouse cell division cycle 20 (Cdc20), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Required for full ubiquitin ligase activity of the anaphase promoting complex/cyclosome (APC/C) and may confer substrate specificity upon the complex. Is regulated by MAD2L1: in metaphase the MAD2L1-CDC20-APC/C ternary complex is inactive and in anaphase the CDC20-APC/C binary complex is active in degrading substrates. The CDC20-APC/C complex positively regulates the formation of synaptic vesicle clustering at active zone to the presynaptic membrane in postmitotic neurons. CDC20-APC/C-induced degradation of NEUROD2 induces presynaptic differentiation.
• Source: HEK293T
• Species: Mouse
• Tag: MYC/DDK
• Molecular Mass: 54.8 kDa
• Purity: >80%, as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 ug/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Cdc20 cell division cycle 20 [ Mus musculus (house mouse) ]
• Official Symbol: Cdc20
• Synonyms: Cdc20; cell division cycle 20; C87100; p55CDC; 2310042N09Rik; cell division cycle protein 20 homolog; cell division cycle 20 homolog; mmCdc20
• Gene ID: 107995
• mRNA Refseq: NM_023223
• Protein Refseq: NP_075712
• UniProt ID: Q9JJ66