Recombinant Mouse Cdk7 Protein, MYC/DDK-tagged
Cat.No. : | Cdk7-868M |
Product Overview : | Purified recombinant protein of full-length mouse cyclin-dependent kinase 7 (homolog of Xenopus MO15 cdk-activating kinase) (cDNA clone MGC:6069 IMAGE:3585145),, with C-terminal MYC/DDK tag, was expressed in HEK293T cells. |
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Description : | Serine/threonine kinase involved in cell cycle control and in RNA polymerase II-mediated RNA transcription. Cyclin-dependent kinases (CDKs) are activated by the binding to a cyclin and mediate the progression through the cell cycle. Each different complex controls a specific transition between 2 subsequent phases in the cell cycle. Required for both activation and complex formation of CDK1/cyclin-B during G2-M transition, and for activation of CDK2/cyclins during G1-S transition (but not complex formation). CDK7 is the catalytic subunit of the CDK-activating kinase (CAK) complex. Phosphorylates SPT5/SUPT5H, SF1/NR5A1, POLR2A, p53/TP53, CDK1, CDK2, CDK4, CDK6 and CDK11B/CDK11. CAK activates the cyclin-associated kinases CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation, thus regulating cell cycle progression. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminal domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts. Phosphorylation of POLR2A in complex with DNA promotes transcription initiation by triggering dissociation from DNA. Its expression and activity are constant throughout the cell cycle. Upon DNA damage, triggers p53/TP53 activation by phosphorylation, but is inactivated in turn by p53/TP53; this feedback loop may lead to an arrest of the cell cycle and of the transcription, helping in cell recovery, or to apoptosis. Required for DNA-bound peptides-mediated transcription and cellular growth inhibition. |
Source : | HEK293T |
Species : | Mouse |
Tag : | MYC/DDK |
Molecular Mass : | 34.6 kDa |
Purity : | >80% as determined by SDS-PAGE and Coomassie blue staining |
Stability : | Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles. |
Storage : | Store at -80 centigrade after receiving vials. |
Concentration : | >50 µg/mL as determined by microplate BCA method |
Storage Buffer : | 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol. |
Gene Name : | Cdk7 cyclin-dependent kinase 7 [ Mus musculus (house mouse) ] |
Official Symbol : | Cdk7 |
Synonyms : | Cdk7; cyclin-dependent kinase 7; Crk4; Cdkn7; AI323415; AI528512; C230069N13; cyclin-dependent kinase 7; 39 kDa protein kinase; CAK; CDK-activating kinase; CR4 protein kinase; CRK4 PK (CDC2-related-kinase-4 protein kinase); P39 Mo15; TFIIH basal transcription factor complex kinase subunit; cell division protein kinase 7; cyclin-dependent kinase 7 (homolog of Xenopus MO15 cdk-activating kinase); protein-tyrosine kinase MPK-7; EC 2.7.11.22; EC 2.7.11.23 |
Gene ID : | 12572 |
mRNA Refseq : | NM_009874 |
Protein Refseq : | NP_034004 |
UniProt ID : | Q03147 |
Products Types
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CDK7-1036H | Active Recombinant Human CDK7 Protein, GST-Tagged | +Inquiry |
CDK7-1035H | Active Recombinant Human CDK7 Protein, GST-His-Tagged | +Inquiry |
◆ Lysates | ||
CDK7-7621HCL | Recombinant Human CDK7 293 Cell Lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (6)
Ask a questionAt present, some drugs have been developed to act on CDK7, including some small molecule inhibitors and antibody drugs. These drugs can inhibit the activity of CDK7 or regulate its expression, thereby treating diseases associated with aberrant expression of CDK7.
Therapeutic strategies for CDK7 include inhibition of its activity, regulation of its expression, and gene therapy. For example, the development of small molecule inhibitors or antibody drugs against CDK7 to inhibit its aberrant activity in tumors, or the regulation of CDK7 expression through gene knockout or overexpression technologies.
The expression of CDK7 is affected by a variety of factors, including transcription factors, epigenetic modifications, and hormones. For example, the E2F transcription factor can promote the transcription of CDK7, while the histone methyltransferase EZH2 can regulate the cell cycle by inhibiting the transcription of CDK7.
Certain biomolecules can be used as molecular markers of CDK7, such as the mRNA or protein of CDK7 detected in biological samples such as blood and urine. These markers can be used for early diagnosis of disease, prognostic judgment, or monitoring of treatment efficacy.
The main role of CDK7 in the cell cycle is to activate CDK2 and CDK1, which in turn drive the cell from G1 to S phase and from G2 to M phase. It regulates cell cycle progression by phosphorylating a variety of substrates, including Rb protein and Wee1 kinase.
CDK7 activity can be detected by methods such as phosphowestern blotting, kinase activity assays, and cell cycle analysis. These methods can detect the phosphorylation level and cell cycle distribution of CDK7 to assess its activity status in cells.
Customer Reviews (3)
Write a reviewWe are very satisfied with the performance and quality of their products and will continue to buy them.
It has high catalysis, high safety and high expression level, which makes the experimental process more convenient and saves a lot of time for scientific research.
The fluorescence and absorption properties are very good.
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