| Description : |
Neuropoietin (NP; also known as cardiotrophin-2) is a 19.7 kDa member of the IL-6 family of cytokines. Considered to be the product of a gene duplication event involving cardiotrophin-1 (CT-1), it helps to define a subfamily within the IL-6 family that includes CT-1, CLC and CTNF. Mouse neuropoietin is synthesized as a 204 amino acid (aa) precursor that contains a 22 aa signal sequence and a 182 aa mature segment. The secreted molecule is characterized by the presence of four alpha-helices, typical of hematopoietic superfamily molecules. Mature murine neuropoietin shares 88%, 90% and 95% aa identity to chimpanzee, canine and rat neuropoietin, respectively. |
| Source : |
E.coli |
| Species : |
Mouse |
| Form : |
Lyophilized from a 0.2μm filtered concentrated solution in 20 mM Tris-HCl, pH8.0, 500 mM NaCl, with 0.5 mM DTT. |
| Bio-activity : |
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 200 ng/ml, corresponding to a specific activity of > 5000 IU/mg. |
| Molecular Mass : |
Approximately 19.7 kDa, a single non-glycosylated polypeptide chain containing 182 amino acids. |
| Protein length : |
182 |
| AA Sequence : |
APISPSEPIGQAYSLALYMQKNTSA LLQTYLQHQGSPFSDPGFSAPELQL STLPSAAVSFKTWHAMEDAERLSRA QGAFLALTQHLQLVGDDQSYLNPGS PILLAQLGAARLRAQGLLGNMAAIM TALGLPIPPEEDTLGFVPFGASAFE RKCRGYIVTREYGHWTDRAVRDLAL LKAKYSA |
| Endotoxin : |
Less than 1 EU/μg of rMuNeuropoietin as determined by LAL method. |
| Purity : |
>98% by SDS-PAGE and HPLC analysis. |
| Storage : |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, -20 to -70 centigrade under sterile conditions after reconstitution. |
| Reconstitution : |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤-20 centigrade. Further dilutions should be made in appropriate buffered solutions. |
