Recombinant Mouse Gars Protein, Myc/DDK-tagged

• Cat.No.: Gars-3154M
• Product Overview: Purified recombinant protein of mouse full-length glycyl-tRNA synthetase (Gars), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Catalyzes the ATP-dependent ligation of glycine to the 3'-end of its cognate tRNA, via the formation of an aminoacyl-adenylate intermediate (Gly-AMP). Also produces diadenosine tetraphosphate (Ap4A), a universal pleiotropic signaling molecule needed for cell regulation pathways, by direct condensation of 2 ATPs. Thereby, may play a special role in Ap4A homeostasis.
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 81.9 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Gars glycyl-tRNA synthetase [ Mus musculus (house mouse) ]
• Official Symbol: Gars
• Synonyms: GARS; glycyl-tRNA synthetase; glycine--tRNA ligase; glyRS; AP-4-A synthetase; storage granule protein 23; diadenosine tetraphosphate synthetase; Nmf249; Sgrp23
• Gene ID: 353172
• mRNA Refseq: NM_180678
• Protein Refseq: NP_851009
• UniProt ID: Q9CZD3