Recombinant Mouse Hsd3b1 Protein, MYC/DDK-tagged

• Cat.No.: Hsd3b1-1182M
• Product Overview: Purified recombinant protein of full-length mouse hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1 (Hsd3b1), with C-terminal MYC/DDK tag, was expressed in HEK293T cells.

Specification

• Description: 3-beta-HSD is a bifunctional enzyme, that catalyzes the oxidative conversion of Delta(5)-ene-3-beta-hydroxy steroid, and the oxidative conversion of ketosteroids. The 3-beta-HSD enzymatic system plays a crucial role in the biosynthesis of all classes of hormonal steroids.
• Source: HEK293T
• Species: Mouse
• Tag: MYC/DDK
• Molecular Mass: 42 kDa
• Purity: >80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 µg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Hsd3b1 hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1 [ Mus musculus (house mouse) ]
• Official Symbol: Hsd3b1
• Synonyms: Hsd3b1; hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1; D3Ertd383e; 3-beta-HSD I; 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1; 3-beta-hydroxy-5-ene steroid dehydrogenase; 3-beta-hydroxy-Delta(5)-steroid dehydrogenase; 3-beta-hydroxysteroid 3-dehydrogenase; delta-5-3-ketosteroid isomerase; dihydrotestosterone oxidoreductase; steroid Delta-isomerase; EC 1.1.1.145; EC 1.1.1.210; EC 1.1.1.270; EC 5.3.3.1
• Gene ID: 15492
• mRNA Refseq: NM_008293
• Protein Refseq: NP_032319
• UniProt ID: P24815