Recombinant Mouse Icoslg protein, Fc-tagged

PRODUCTS

Home / Products / Recombinant Proteins / Recombinant Mouse Icoslg protein, Fc-tagged

Recombinant Mouse Icoslg protein, Fc-tagged

Icoslg Related Products

Price Inquiry

Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.

Cat.No. : Icoslg-877M
Product Overview : Recombinant Mouse Icoslg (Glu47-Lys279) protein was fused to human IgG1 Fc tag at C-terminus and expressed in human 293 cells (HEK293).
Description : ICOS ligand (ICOSLG) is also known as B7 homolog 2 (B7-H2), B7-related protein 1 (B7RP-1) and CD antigen CD275, which belongs to the immunoglobulin superfamily and BTN/MOG family. ICOSLG contains one Ig-like C2-type (immunoglobulin-like) domain and one Ig-like V-type (immunoglobulin-like) domain. Isoform 1 is widely expressed, while isoform 2 is detected only in lymph nodes, leukocytes and spleen. B7-H2 is ligand for the T-cell-specific cell surface receptor ICOS. B7-H2 acts as a costimulatory signal for T-cell proliferation and cytokine secretion and induces also B-cell proliferation and differentiation into plasma cells. B7-H2 could play an important role in mediating local tissue responses to inflammatory conditions, as well as in modulating the secondary immune response by co-stimulating memory T-cell function.
Source : HEK293
Species : Mouse
Tag : human IgG1 Fc
Predicted N Terminal : Glu47
Form : Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5, 10% trehalose.
Molecular Mass : The protein has a calculated MW of 52.7 kDa. The protein migrates as 65-80 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Protein length : Glu47-Lys279
Endotoxin : Less than 1.0 EU per μg by the LAL method.
Purity : >95% as determined by SDS-PAGE.
Storage : For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20 to -70 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
SDS-PAGE of Icoslg-877M :

Mouse B7-H2, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA of Icoslg-877M :

Immobilized Mouse ICOS (C137S, C138S), His Tag at 5 μg/mL (100 μL/well) can bind Mouse B7-H2, Fc Tag with a linear range of 2-31 ng/mL (QC tested).

Gene Name : Icoslg
Official Symbol : Icosl
Synonyms : ICOSL; icos ligand; ICOS ligand; B7 homolog 2; B7-like protein Gl50; B7-related protein 1; B7h; GI50; GL50; B7-H2; LICOS; B7RP-1; GL50-B; ICOS-L; Icoslg; Ly115l; AU044799; BG071784; mKIAA0653; KIAA0653
Gene ID : 50723
mRNA Refseq : NM_015790
Protein Refseq : NP_056605
UniProt ID : Q9JHJ8

Online Inquiry


  • Note: There will be extra charge for optional service!
  • Please input "biomart" as verification code. Please review Creative BioMart's privacy policy for more information

Optional Service: Optional requirements on this protein

Other Requirements:

Apply For A Coupon

$50 OFF Your First Purchase

Apply For a Coupon

Enter your email here to subscribe.

creative biomart inc.

Easy access to products and services you need from our library via powerful searching tools.

Follow Us

Copyright © 2007 – 2020 Creative BioMart. All Rights Reserved.