Recombinant Mouse Icoslg protein, Fc-tagged


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Recombinant Mouse Icoslg protein, Fc-tagged

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Cat.No. : Icoslg-877M
Product Overview : Recombinant Mouse Icoslg (Glu47-Lys279) protein was fused to human IgG1 Fc tag at C-terminus and expressed in human 293 cells (HEK293).
Description : ICOS ligand (ICOSLG) is also known as B7 homolog 2 (B7-H2), B7-related protein 1 (B7RP-1) and CD antigen CD275, which belongs to the immunoglobulin superfamily and BTN/MOG family. ICOSLG contains one Ig-like C2-type (immunoglobulin-like) domain and one Ig-like V-type (immunoglobulin-like) domain. Isoform 1 is widely expressed, while isoform 2 is detected only in lymph nodes, leukocytes and spleen. B7-H2 is ligand for the T-cell-specific cell surface receptor ICOS. B7-H2 acts as a costimulatory signal for T-cell proliferation and cytokine secretion and induces also B-cell proliferation and differentiation into plasma cells. B7-H2 could play an important role in mediating local tissue responses to inflammatory conditions, as well as in modulating the secondary immune response by co-stimulating memory T-cell function.
Source : HEK293
Species : Mouse
Tag : human IgG1 Fc
Predicted N Terminal : Glu47
Form : Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5, 10% trehalose.
Molecular Mass : The protein has a calculated MW of 52.7 kDa. The protein migrates as 65-80 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Protein length : Glu47-Lys279
Endotoxin : Less than 1.0 EU per μg by the LAL method.
Purity : >95% as determined by SDS-PAGE.
Storage : For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20 to -70 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
SDS-PAGE of Icoslg-877M :

Mouse B7-H2, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA of Icoslg-877M :

Immobilized Mouse ICOS (C137S, C138S), His Tag at 5 μg/mL (100 μL/well) can bind Mouse B7-H2, Fc Tag with a linear range of 2-31 ng/mL (QC tested).

Gene Name : Icoslg
Official Symbol : Icosl
Synonyms : ICOSL; icos ligand; ICOS ligand; B7 homolog 2; B7-like protein Gl50; B7-related protein 1; B7h; GI50; GL50; B7-H2; LICOS; B7RP-1; GL50-B; ICOS-L; Icoslg; Ly115l; AU044799; BG071784; mKIAA0653; KIAA0653
Gene ID : 50723
mRNA Refseq : NM_015790
Protein Refseq : NP_056605
UniProt ID : Q9JHJ8

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