Active Recombinant Mouse Il10 protein, His-tagged

• Cat.No.: Il10-501M
• Product Overview: Active Recombinant Mouse Il10 protein(P18893)(19~178 aa), fused with N-terminal His tag, was expressed in HEK293.

Specification

• Species: Mouse
• Source: HEK293
• Tag: His
• Protein Length: 19-178 aa
• Form: Lyophilized from sterile 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Bio-activity: Interleukin 10 (IL-10), also known as human cytokine synthesis inhibitory factor (CSIF), is an anti-inflammatory cytokine. IL-10 is a cytokine with multiple, pleiotropic, effects in immunoregulation and inflammation. It downregulates the expression of Th1 cytokines, MHC class II antigens, and co-stimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. IL-10 can block NF-κB activity, and is involved in the regulation of the JAK-STAT signaling pathway. Besides, Interleukin 10 Receptor Alpha (IL10Ra) has been identified as an interactor of IL-10, thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse IL-10 and recombinant mouse IL10Ra. Briefly, IL-10 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100μL were then transferred to IL10Ra-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-IL-10 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of IL-10 and IL10Ra was shown, and this effect was in a dose dependent manner.
• Molecular Mass: 20.4kDa
• Endotoxin: <1.0EU per 1μg (determined by the LAL method).
• Purity: > 97% as determined by SDS-PAGE.
• Storage: Avoid repeated freeze/thaw cycles. Store at -20°C for 12 months. Aliquot and store at -80°C for 12 months.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (PH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: Il10 interleukin 10 [ Mus musculus ]
• Official Symbol: Il10
• Synonyms: IL10; interleukin 10; interleukin-10; cytokine synthesis inhibitory factor; CSIF; Il-10
• Gene ID: 16153
• mRNA Refseq: NM_010548
• Protein Refseq: NP_034678