Recombinant Human IL12RB2 protein, His-tagged

Cat.No. : IL12RB2-3885H
Product Overview : Recombinant Human IL12RB2 protein is produced by Baculovirus-Insect Cells expression system and is fused with a His tag at the C-terminal. A DNA sequence encoding the human IL12RB2 (NP_001245143.1) (Met1-Asn622) was expressed with a polyhistidine tag at the C-terminus.
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  • Gene Information
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Source : Baculovirus-Insect Cells
Species : Human
Tag : His
Form : Lyophilized from sterile 20 mM PB, 150 mM NaCl, pH 7.0, 10 % glycerol, 1 mM TCEP. Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization.
Molecular Mass : 69.2 kDa
Protein length : Met1-Asn622
Endotoxin : Less than 1.0 EU per ug by the LAL method.
Purity : > 90 % as determined by SDS-PAGE.
Stability : Samples are stable for up to twelve months from date of receipt at -70 centigrade.
Storage : Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25mg/ml.
Shipping : In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Gene Name : IL12RB2 interleukin 12 receptor, beta 2 [ Homo sapiens ]
Official Symbol : IL12RB2
Synonyms : IL12RB2; IL-12RB2; IL-12R-beta-2; RP11-102M16.1;
Gene ID : 3595
mRNA Refseq : NM_001258214
Protein Refseq : NP_001245143
MIM : 601642
UniProt ID : Q99665

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (10)

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Could you explain the benefits of using CRISPR/Cas9-mediated gene tagging to track IL12RB2 localization? 12/04/2022

CRISPR/Cas9 gene tagging tracks IL12RB2 localization dynamically.

What methods are suitable for assessing IL12RB2 dimerization and its effect on downstream signaling? 10/23/2022

Crosslinking and immunoprecipitation study IL12RB2 dimerization.

How can ChIP-seq analysis provide insights into the genes regulated by IL12RB2? 06/30/2022

ChIP-seq identifies genes under IL12RB2's regulation.

How does quantitative RT-PCR validate IL12RB2's impact on cytokine-induced gene expression? 05/24/2022

Quantitative RT-PCR validates IL12RB2's impact on gene expression.

What experimental approaches can validate IL12RB2's involvement in signal transduction pathways? 04/01/2022

Functional assays validate IL12RB2's role in signaling pathways.

What are the advantages of utilizing flow cytometry to analyze cell-specific IL12RB2 expression? 03/26/2022

Flow cytometry quantifies IL12RB2 expression on specific cell types.

Can you elaborate on the use of confocal microscopy to visualize the subcellular distribution of IL12RB2? 11/22/2021

Confocal microscopy visualizes IL12RB2 distribution in cells.

How can siRNA knockdown experiments reveal the functional role of IL12RB2? 02/04/2021

siRNA knockdown reveals IL12RB2's functional significance.

How does co-immunoprecipitation assist in understanding the protein-protein interactions of IL12RB2? 01/15/2021

Co-immunoprecipitation unveils IL12RB2's protein interactions.

What techniques are effective in studying IL12RB2's interaction with cytokines IL12A and IL23A? 04/06/2019

Co-immunoprecipitation and ELISA assay cytokine interactions with IL12RB2.

Customer Reviews (3)

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Reviews
08/14/2022

    Good for receptor binding studies.

    03/17/2022

      Consistent in bioactivity.

      07/31/2017

        Suitable for flow cytometry.

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