Active Recombinant Human IL12RB2 protein, His-tagged

• Cat.No.: IL12RB2-3233H
• Product Overview: Recombinant Human IL12RB2 protein(Q99665-1)(Lys 24 - Asn 622), fused with His Tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: His
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
• Bio-activity: Immobilized Human IL-12 R beta 2, His Tag at 5 μg/mL (100 μL/well) can bind Biotinylated Human IL-12B&IL-12A Heterodimer, His,Avitag&Flag Tag with a linear range of 0.01-0.3 μg/mL.
• Molecular Mass: The protein has a calculated MW of 70.0 kDa. The protein migrates as 66 kDa and 80-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Protein Length: Lys 24 - Asn 622
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term storage, the product should be stored at lyophilized state at -20°C or lower.
  Please avoid repeated freeze-thaw cycles.
  This product is stable after storage at:
  -20°C to -70°C for 12 months in lyophilized state;
  -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: IL12RB2 interleukin 12 receptor, beta 2 [ Homo sapiens ]
• Official Symbol: IL12RB2
• Synonyms: IL12RB2; interleukin 12 receptor, beta 2; interleukin-12 receptor subunit beta-2; IL-12RB2; IL-12R-beta-2; IL-12 receptor beta 2; IL-12R subunit beta-2; IL-12 receptor subunit beta-2; interleukin-12 receptor beta-2 chain; RP11-102M16.1
• Gene ID: 3595
• mRNA Refseq: NM_001258214
• Protein Refseq: NP_001245143
• MIM: 601642
• UniProt ID: Q99665

Reviews

08/14/2022

Good for receptor binding studies.

03/17/2022

Consistent in bioactivity.

07/31/2017

Suitable for flow cytometry.

Q&As

Could you explain the benefits of using CRISPR/Cas9-mediated gene tagging to track IL12RB2 localization?

CRISPR/Cas9 gene tagging tracks IL12RB2 localization dynamically.

What methods are suitable for assessing IL12RB2 dimerization and its effect on downstream signaling?

Crosslinking and immunoprecipitation study IL12RB2 dimerization.

How can ChIP-seq analysis provide insights into the genes regulated by IL12RB2?

ChIP-seq identifies genes under IL12RB2's regulation.

How does quantitative RT-PCR validate IL12RB2's impact on cytokine-induced gene expression?

Quantitative RT-PCR validates IL12RB2's impact on gene expression.

What experimental approaches can validate IL12RB2's involvement in signal transduction pathways?

Functional assays validate IL12RB2's role in signaling pathways.

What are the advantages of utilizing flow cytometry to analyze cell-specific IL12RB2 expression?

Flow cytometry quantifies IL12RB2 expression on specific cell types.

Can you elaborate on the use of confocal microscopy to visualize the subcellular distribution of IL12RB2?

Confocal microscopy visualizes IL12RB2 distribution in cells.

How can siRNA knockdown experiments reveal the functional role of IL12RB2?

siRNA knockdown reveals IL12RB2's functional significance.

How does co-immunoprecipitation assist in understanding the protein-protein interactions of IL12RB2?

Co-immunoprecipitation unveils IL12RB2's protein interactions.

What techniques are effective in studying IL12RB2's interaction with cytokines IL12A and IL23A?

Co-immunoprecipitation and ELISA assay cytokine interactions with IL12RB2.