Recombinant Mouse IL17RA protein(Met1-Trp322), hFc-tagged

• Cat.No.: IL17RA-404M
• Product Overview: Recombinant Mouse IL17RA protein(Q60943)(Met1-Trp322) was expressed in HEK293 with the Fc region of human IgG1 at the C-terminus.

Specification

• Species: Mouse
• Source: HEK293
• Tag: Fc
• Protein Length: 1-322 a.a.
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized mouse IL17A at 10 μg/ml (100 μl/well) can bind mouse IL17RA-Fc, The EC50 of mouse IL17RA-Fc is 0.28-0.64 μg/ml.
• Molecular Mass: The recombinant mouse IL17RA/Fc is a disulfide-linked homodimer. The reduced monomer comprises 532 amino acids and has a predicted molecular mass of 60.3 kDa. The apparent molecular mass of the protein is approximately 67-77 and 118 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 90 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Il17ra interleukin 17 receptor A [ Mus musculus ]
• Official Symbol: IL17RA
• Synonyms: IL17RA; interleukin 17 receptor A; interleukin-17 receptor A; IL-17RA; IL-17 receptor A; Il17r; Cdw217; VDw217; AW538159
• Gene ID: 16172
• mRNA Refseq: NM_008359
• Protein Refseq: NP_032385