Recombinant Mouse Il18 protein, His-tagged

• Cat.No.: Il18-5440M
• Product Overview: Recombinant Mouse Il18 protein(P70380)(1-192aa), fused to N-terminal His tag, was expressed in Yeast.

Specification

• Source: Yeast
• Species: Mouse
• Tag: His
• Form: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
• Molecular Mass: 24.6 kDa
• Protein length: 1-192aa
• Purity: Greater than 90% as determined by SDS-PAGE.
• Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Reconstitution: Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%.

Gene Information

• Gene Name: Il18 interleukin 18 [ Mus musculus ]
• Official Symbol: Il18
• Synonyms: IL18; interleukin 18; interleukin-18; IL-1 gamma; interleukin-1 gamma; IFN-gamma-inducing factor; interferon gamma-inducing factor; interferon-gamma-inducing factor; Igif; Il-18
• Gene ID: 16173
• mRNA Refseq: NM_008360
• Protein Refseq: NP_032386

Reviews

10/15/2022

Effective in inflammation models.

08/22/2021

High stability in serum.

05/09/2019

Suitable for ELISA.

Q&As

How do researchers experimentally investigate the crosstalk between IL18 and other immune signaling pathways, such as Toll-like receptor signaling?

Co-immunoprecipitation, co-localization studies, and pathway inhibition assays decipher IL18's interactions with signaling molecules and pathways.

What assays and methodologies are employed to evaluate the potential therapeutic interventions targeting IL18 in autoimmune diseases and chronic inflammation?

Therapeutic antibody trials, in vitro drug screens, and disease model assessments assess IL18-targeted interventions in autoimmune diseases.

How are animal models and in vivo imaging techniques used to understand the kinetics of IL18-mediated immune responses and its tissue-specific effects?

Xenograft models, intravital imaging, and tissue-specific knockout approaches unravel IL18's kinetics and effects within complex in vivo environments.

Can you elaborate on the techniques used to assess the impact of IL18 on immune cell activation, migration, and effector functions?

Flow cytometry, chemotaxis assays, and functional cell assays examine IL18's impact on immune cell activation, migration, and effector functions.

Can you explain the experimental strategies used to analyze the interactions between IL18 and its receptor complex, as well as the downstream signaling events triggered by receptor engagement?

Surface plasmon resonance, mutational studies, and phosphoproteomics illuminate IL18's receptor interactions and downstream signal transduction pathways.

How are cellular and molecular techniques employed to investigate IL18's role in regulating the balance between Th1 and Th2 immune responses?

Immunophenotyping, cytokine profiling, and gene expression analyses elucidate IL18's influence on Th cell polarization and immune balance.

What experimental approaches are utilized to study the genetic and epigenetic regulation of IL18 expression in response to different stimuli?

ChIP assays, promoter analyses, and epigenetic profiling uncover the regulatory mechanisms underlying IL18 gene expression dynamics.

What methods are used to dissect the mechanisms through which IL18 contributes to the pathogenesis of inflammatory diseases and its interactions with other proinflammatory molecules?

Disease models, knockout mice, and co-stimulation studies explore IL18's involvement in inflammatory conditions and its interplay with other proinflammatory mediators.

How do researchers investigate the role of IL18 in modulating the tumor microenvironment and its potential implications for cancer immunotherapy?

Tumor models, immune profiling, and immunotherapeutic studies investigate IL18's modulation of the tumor microenvironment and its implications for cancer therapy.

Could you explain the experimental strategies used to characterize the inflammasome activation triggered by IL18 and its downstream effects on cytokine production?

Caspase assays, cytokine ELISAs, and microscopy techniques unveil the activation of inflammasomes and IL18's contribution to pyroptosis and cytokine storms.