Active Recombinant Human Interleukin 36 Receptor Antagonist

• Cat.No.: IL36RN-5660H
• Product Overview: RecombinantHuman IL36RN protein was expressed in E. coli. Val2-Asp155.

Specification

• Cat. No.: IL36RN-5660H
• Description: IL36RN isa member of the IL-1 family of proteins. IL-1 family members include IL-1β,IL-1α, IL-1ra, IL-18 and IL-1F5-F10.All family members show a 12 β-strand,β-trefoil configuration, and all family members are believed to have arisenfrom a common ancestral gene that underwent multiple duplications. The human IL-36Ra/IL-1F5gene is in closest proximity to the gene for IL-1ra and is likely arelatively recent duplication of the IL-1ra gene.
• Source: E. coli
• Form: Lyophilizedfrom a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• N-terminalSequence: Val2
• MolecularWeight: 17 kDa
• Activity: Measuredby its ability to inhibit IL-36α, IL-36β or IL-36γ-induced IL-8 secretion by humanpreadipocytes. The ED50 for this effect is typically 0.25-1.25 μg/mL in thepresence of 20 ng/mL of recombinant human IL-36β. Measured by its bindingability in a functional ELISA. Immobilized recombinant human IL-36Ra at 1μg/mL (100 μL/well) can bind recombinant human IL-1 Rrp2 Fc Chimera with alinear range of 0.15-5 μg/mL.
• Endotoxin Level: <1.0EU per 1μg of the protein by the LAL method.
• SDS-PAGE: 65 kDa,reducing conditions.
• Purity: >90%,by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution: 100 μg/mLin sterile PBS
• Storage: Use amanual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied.1 month, 2 to 8°C under sterile conditions after reconstitution. 3months, -20 to -70°C under sterile conditions after reconstitution.

Gene Information

• Gene Name: IL36RN interleukin 36 receptorantagonist [ Homo sapiens ]
• Official Symbol: IL36RN
• Synonyms: IL36RN; interleukin36 receptor antagonist; FIL1; FIL1D; IL1F5; IL1L1; PSORP; IL1HY1;IL1RP3; IL36RA; FIL1 (DELTA); interleukin-36 receptor antagonist protein; IL-1rahomolog 1; interleukin-1 HY1; IL-1 related protein 3; interleukin-1-likeprotein 1; family of interleukin 1-delta; IL1F5 (Canonical product IL-1F5a);interleukin 1 family, member 5 (delta); interleukin-1 receptor antagonisthomolog 1; IL-1F5 (IL-1HY1, FIL1-delta, IL-1RP3, IL-1L1, IL-1-delta)
• Gene ID: 26525
• mRNA Refseq: NM_012275
• Protein Refseq: NP_036407
• MIM: 605507
• UniProt ID: Q9UBH0
• Chromosome Location: 2q14
• Function: cytokine activity;interleukin-1 receptor antagonist activity

Reviews

05/31/2021

When conducting experiments with IL36RN, clear and consistent results can be obtained, facilitating data analysis and interpretation.

01/29/2019

IL36RN has high specific activity and can exert strong biological effect at low concentration.

01/11/2019

The stability of the IL36RN product allows it to cope with different experimental conditions and modes of operation.

Q&As

What is the action mechanism of IL36RN?

The mechanism of action of IL36RN is to play an anti-inflammatory, anti-infection, anti-tumor and other biological mechanisms by acting on the signaling pathway of IL-36 receptor.

How to ensure the purity of IL36RN when preparing it?

The purity of IL36RN can be ensured by ion exchange chromatography, dialysis, gel filtration and other methods.

IL36RN production process is what?

Mass production of IL36RN can be carried out through large-scale fermentation, purification and other processes.

What is the difference between IL36RN protein and IL36α, β, γ?

This protein is an inhibitor with no biological activity of its own; Recombinant IL36α, β and γ are agonists with different biological effects.

Can recombinant IL36RN be used in clinical trials and therapeutics?

Can be used in clinical trials and therapy.

Does IL36RN have sustained and stable biological activity?

IL36RN has sustained and stable biological activity.