Recombinant Mouse IL23A protein, His-tagged

Cat.No. : IL23A-159M
Product Overview : Recombinant Mouse IL23A (Q9EQ14) (Met1-Ala196 ) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1; Recombinant Mouse IL12B (P43432) (Met1-Ser335) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the mouse IL23 heterodimer was purified in HEK293.
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Source : HEK293
Species : Mouse
Tag : His
Protein length : Met1-Ala196&Met1-Ser335
Form : Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Bio-activity : 1. Immobilized Human IL23 Receptor hFc at 2 μg/ml (100 μl/well) can bind Mouse IL-23 His, the EC50 of Mouse IL-23 His is 50-250 ng/mL. 2. Measured by its ability to induce IL17 secretion by mouse splenocytes. The ED50 for this effect is 0.05-1 ng/mL.
Molecular Mass : The recombinant mouse IL23 (mouse IL23A&IL12B Heterodimer) comprises 510 (186+324) amino acids and has a calculated molecular mass of 58.3 (21.1+ 37.2) KDa. The apparent molecular mass of the recombinant protein is approximately 47,45 and 26 KDa respectively in SDS-PAGE under reducing conditions.
Endotoxin : < 1.0 EU per μg of the protein as determined by the LAL method
Purity : > 95 % as determined by SDS-PAGE
Storage : Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
Gene Name : Il23a interleukin 23, alpha subunit p19 [ Mus musculus ]
Official Symbol : IL23A
Synonyms : IL23A; interleukin 23, alpha subunit p19; interleukin-23 subunit alpha; IL-23-A; IL-23p19; IL-23 subunit alpha; interleukin-23 subunit p19; p19; IL-23;
Gene ID : 83430
mRNA Refseq : NM_031252
Protein Refseq : NP_112542

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Customer Reviews (3)

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Reviews
04/18/2020

    Coupled with the manufacturer's exceptional technical support and commitment to customer satisfaction, researchers can confidently utilize the IL23A protein in their studies, knowing that they have the necessary resources and assistance to succeed.

    12/02/2016

      Its remarkable attributes and consistent results make it an indispensable tool in various experimental applications.

      02/25/2016

        Highly recommended for its exceptional performance in ELISA, the IL23A protein is a top contender for researchers involved in protein electron microscopy structure analysis.

        Q&As (6)

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        Is this bioactive cyno IL-23 heterodimer (p19/p40) or just the cyno IL-23p19 subunit? 02/01/2023

        Please inquiry our product manager with specific product.

        Are there any FDA-approved drugs targeting IL23A? 12/22/2021

        Yes, drugs like ustekinumab and risankizumab, which target IL23A, have been approved for certain autoimmune diseases.

        Is IL23A involved in the regulation of adaptive or innate immunity? 08/23/2018

        IL23A primarily plays a role in regulating the adaptive immune response.

        How is IL23A linked to the pathogenesis of ankylosing spondylitis? 01/25/2018

        IL23A is associated with the inflammation of the spine and joints seen in ankylosing spondylitis, making it a target for therapeutic intervention.

        How does IL23A contribute to rheumatoid arthritis? 09/01/2017

        In rheumatoid arthritis, IL23A is involved in the dysregulation of the immune system, leading to joint inflammation and damage.

        How is IL23A involved in inflammatory bowel diseases like Crohn's disease? 07/03/2016

        IL23A is implicated in the pathogenesis of Crohn's disease, contributing to chronic inflammation in the gastrointestinal tract.

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