Recombinant Mouse Ilk Protein, Myc/DDK-tagged

• Cat.No.: Ilk-3525M
• Product Overview: Purified recombinant protein of mouse full-length integrin linked kinase (Ilk), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Receptor-proximal protein kinase regulating integrin-mediated signal transduction. May act as a mediator of inside-out integrin signaling. Focal adhesion protein part of the complex ILK-PINCH. This complex is considered to be one of the convergence points of integrin- and growth factor-signaling pathway. Could be implicated in mediating cell architecture, adhesion to integrin substrates and anchorage-dependent growth in epithelial cells. Phosphorylates beta-1 and beta-3 integrin subunit on serine and threonine residues, but also AKT1 and GSK3B.
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 51.4 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Ilk integrin linked kinase [ Mus musculus (house mouse) ]
• Official Symbol: Ilk
• Synonyms: ILK; integrin linked kinase; integrin-linked protein kinase; integrin binding protein kinase; ESTM24; AA511515
• Gene ID: 16202
• mRNA Refseq: NM_010562
• Protein Refseq: NP_034692
• UniProt ID: O55222