| Description : |
N6-adenine-specific methyltransferase that can methylate both RNAs and DNA. Acts as a N6-adenine-specific RNA methyltransferase by catalyzing formation of N6,2'-O-dimethyladenosine (m6A(m)) on internal positions of U2 small nuclear RNA (snRNA): methylates the 6th position of adenine residues with a pre-deposited 2'-O-methylation. Internal m6A(m) methylation of snRNAs regulates RNA splicing. Also able to act as a N6-adenine-specific DNA methyltransferase by mediating methylation of DNA on the 6th position of adenine (N6-methyladenosine). The existence of N6-methyladenosine (m6A) on DNA is however unclear in mammals, and additional evidences are required to confirm the role of the N6-adenine-specific DNA methyltransferase activity of METTL4 in vivo. Acts as a regulator of mitochondrial transcript levels and mitochondrial DNA (mtDNA) copy number by mediating mtDNA N6-methylation: m6A on mtDNA reduces transcription by repressing TFAM DNA-binding and bending. N6-methyladenosine deposition by METTL4 regulates Polycomb silencing by triggering ubiquitination and degradation of sensor proteins ASXL1 and MPND, leading to inactivation of the PR-DUB complex and subsequent preservation of Polycomb silencing. |
| Source : |
HEK293T |
| Species : |
Mouse |
| Tag : |
Myc/DDK |
| Molecular Mass : |
53.3 kDa |
| Purity : |
> 80% as determined by SDS-PAGE and Coomassie blue staining |
| Stability : |
Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles. |
| Storage : |
Store at -80 centigrade after receiving vials. |
| Concentration : |
>50 μg/mL as determined by microplate BCA method |
| Storage Buffer : |
25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol. |
