"Osm" Related Products

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Recombinant Mouse Osm Protein, His-tagged

Cat.No.: Osm-841M
Product Overview: Recombinant mature form of mouse OSM (P53347) (Met 1-Arg 206) was expressed, with a C-terminal polyhistidine tag.
Source: HEK293
Species: Mouse
Tag: His
Predicted N Terminal: Asn 25
Form: Lyophilized from sterile PBS, pH 7.4, 5%~8% trehalose and mannitol.
Bio-activity: 1. Measured by its ability to inhibit the proliferation of M1 mouse myeloid leukemia cells. The ED50 for this effect is typically 50-200 ng/mL. 2. Measured in a cell proliferation assay using NIH3T3 cells. The ED50 for this effect is typically 0.8-4 ng/mL
Molecular Mass: The secreted recombinant mouse OSM comprises 193 amino acids and has a calculated molecular mass of 22 kDa. As a result of glycosylation, the apparent molecular mass of the recombinant protein is approximately 35-40 kDa in SDS-PAGE under reducing conditions.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Purity: >95 % as determined by SDS-PAGE.
Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
Gene Name: Osm oncostatin M [ Mus musculus ]
Official Symbol: Osm
Synonyms: OSM; oncostatin M; oncostatin-M; OncoM;
Gene ID: 18413
mRNA Refseq: NM_001013365
Protein Refseq: NP_001013383
MIM:
UniProt ID:
Pathway: Adipogenesis, organism-specific biosystem; Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; Jak-STAT signaling pathway, organism-specific biosystem; Jak-STAT signaling pathwa
Function: cytokine activity; cytokine activity; growth factor activity; oncostatin-M receptor binding;

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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