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Recombinant Mouse Podxl Protein, His-tagged

Cat.No.: Podxl-31M
Product Overview: Recombinant mouse PODXL, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
Description: PODXL, also known as Podocalyxin, is a heavily glycosylated transmembrane sialoprotein in the CD34 and endoglycan family. This protein involved in the regulation of both adhesion and cell morphology and cancer progression. It has to function as an anti-adhesive molecule that maintains an open filtration pathway between neighboring foot processes in the podocyte by charge repulsion. Also, it acts as a pro-adhesive molecule, enhancing the adherence of cells to immobilized ligands, increasing the rate of migration and cell-cell contacts in an integrin-dependent manner.
Source: Insect cells
Species: Mouse
Tag: His tag
Form: Liquid. In Phosphate Buffered Saline (pH 7.4) containing 10% glycerol.
Molecular Mass: 41.0kDa (391aa)70-100KDa (SDS-PAGE under reducing conditions.)
AA Sequence: HNGNETSTSA IKSSTVQSHQ SATTSTEVTT GHPVASTLAS TQPSNPTPFT TSTQSPSMPT STPNPTSNQS GGNLTSSVSE VDKTKTSSPS STAFTSSSGQ TASSGGKSGD SFTTAPTTTL GLINVSSQPT DLNTTSKLLS TPTTDNTTSP QQPVDSSPST ASHPVGQHTP AAVPSSSGST PSTDNSTLTW KPTTHKPLGT SEATQPLTSQ TPGITTLPVS TLQQSMASTV GTTTEEFTHL ISNGTPVAPP GPSTPSPIWA FGNYQLNCEP PIRPDEELLI LNLTRASLCE RSPLDEKEKL VELLCHSVKA SFKPAEDLCT LHVAPILDNQ AVAVKRIIIE TKLSPKAVYE LLKDRWDDLT EAGVSDMKLG KEGPPEVNED RFSLEHHHHH H
Endotoxin: < 1.0 EU per 1 microgram of protein (determined by LAL method)
Purity: >95% by SDS - PAGE
Storage: Can be stored at +4centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20Centigrade or -70Centigrade. Avoid repeated freezing and thawing cycles.
Concentration: 0.5mg/ml (determined by Absorbance at 280nm)
Gene Name: Podxl podocalyxin-like [ Mus musculus ]
Official Symbol: Podxl
Synonyms: PODXL; podocalyxin-like; podocalyxin; PC; Ly102; Pclp1; PCLP-1; AW121214;
Gene ID: 27205
mRNA Refseq: NM_013723
Protein Refseq: NP_038751
MIM:
UniProt ID:

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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