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Recombinant Mouse Sema7a protein, Fc-tagged

Cat.No.: Sema7a-191M
Product Overview: Recombinant Mouse Sema7a(Gln45-Ala646) fused with Fc of Mouse IgG2A(Glu98-Lys330) at C-terminal was expressed in NS0.
Source: NS0
Species: Mouse
Tag: Fc
Predicted N Terminal: Gln45 predicted
Form: Lyophilized from a 0.2 μm filtered solution in PBS.
Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of THP‑1 human acute monocytic leukemia cells. Suzuki, K. et al. (2007) Nature 446:680. The ED50 for this effect is typically 0.1-0.5 μg/mL.
Molecular Mass: Predicted Molecular Mass: 95.5 (monomer) kDaSDS-PAGE: 100-110 kDa, reducing conditions
Protein length: Gln45-Ala646
Endotoxin: <0.10 EU per 1 μg of the protein by the LAL method.
Purity: >85%, by SDS-PAGE under reducing conditions and visualized by silver stain
Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 centigrade as supplied.1 month, 2 to 8 centigrade under sterile conditions after reconstitution.3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution: Reconstitute at 500 μg/mL in PBS.
Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Gene Name: Sema7a sema domain, immunoglobulin domain (Ig), and GPI membrane anchor, (semaphorin) 7A [ Mus musculus ]
Official Symbol: Sema7a
Synonyms: SEMA7A; sema domain, immunoglobulin domain (Ig), and GPI membrane anchor, (semaphorin) 7A; semaphorin-7A; sema L; sema K1; H-Sema K1; semaphorin-L; Semaphorin K1; semaphorin-K1; Semal; CDw108; H-Sema-L; M-Sema-L; 2900057C09Rik;
Gene ID: 20361
mRNA Refseq: NM_011352
Protein Refseq: NP_035482
MIM:
UniProt ID: Q9QUR8
Chromosome Location: 9; 9 A3.3-B
Pathway: Axon guidance, organism-specific biosystem; Axon guidance, conserved biosystem; Axon guidance, organism-specific biosystem; Developmental Biology, organism-specific biosystem; Other semaphorin interactions, organism-specific biosystem; Semaphorin interactions, organism-specific biosystem;
Function: integrin binding; receptor activity;

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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