Recombinant Mouse Skp2 Protein, Myc/DDK-tagged

• Cat.No.: Skp2-5895M
• Product Overview: Purified recombinant protein of mouse full-length S-phase kinase-associated protein 2 (p45) (Skp2), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Substrate recognition component of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell cycle progression, signal transduction and transcription. The SCF complex provides substrate specificity and interacts with both, the E2 ubiquitin-conjugating enzyme and the substrate. Specifically recognizes phosphorylated CDKN1B/p27kip and is involved in regulation of G1/S transition. Degradation of CDKN1B/p27kip also requires CKS1. Promotes ubiquitination and destruction of CDH1 in a CK1-Dependent Manner, thereby regulating cell migration.
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 48.2 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Skp2 S-phase kinase-associated protein 2 [ Mus musculus (house mouse) ]
• Official Symbol: Skp2
• Synonyms: SKP2; S-phase kinase-associated protein 2 (p45); S-phase kinase-associated protein 2; p45; FWD1; F-box protein Skp2; F-box/WD-40 protein 1; cyclin A/CDK2-associated protein p45; cyclin-A/CDK2-associated protein p45; FBXL1; MGC102075; MGC116668
• Gene ID: 27401
• mRNA Refseq: NM_013787
• Protein Refseq: NP_038815
• UniProt ID: Q9Z0Z3