Recombinant Mouse Smad7 Protein, Myc/DDK-tagged

• Cat.No.: Smad7-5954M
• Product Overview: Purified recombinant protein of mouse full-length SMAD family member 7 (Smad7), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Antagonist of signaling by TGF-beta (transforming growth factor) type 1 receptor superfamily members; has been shown to inhibit TGF-beta (Transforming growth factor) and activin signaling by associating with their receptors thus preventing SMAD2 access. Functions as an adapter to recruit SMURF2 to the TGF-beta receptor complex. Also acts by recruiting the PPP1R15A-PP1 complex to TGFBR1, which promotes its dephosphorylation. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 46.4 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Smad7 SMAD family member 7 [ Mus musculus (house mouse) ]
• Official Symbol: Smad7
• Synonyms: SMAD7; MAD homolog 7 (Drosophila); mothers against decapentaplegic homolog 7; madh8; SMAD 7; MAD homolog 8; SMAD family member 7; mothers against DPP homolog 7; mothers against DPP homolog 8; mothers against decapentaplegic homolog 8; Madh7
• Gene ID: 17131
• mRNA Refseq: NM_001042660
• Protein Refseq: NP_001036125
• UniProt ID: O35253