Recombinant Mouse Sphk1 Protein, Myc/DDK-tagged

• Cat.No.: Sphk1-6092M
• Product Overview: Purified recombinant protein of mouse full-length sphingosine kinase 1 (Sphk1), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: This gene encodes a kinase that phosphorylates sphingosine into sphingosine-1-phosphate, which is involved in cell differentiation, motility, and apoptosis. The encoded protein plays a role in maintaining cellular levels of sphingosine-1-phosphate. Alternative splicing results in multiple transcript variants.
• Source: HEK293T
• Species: Mouse
• Tag: Myc&DDK
• Molecular Mass: 43.7 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Sphk1 sphingosine kinase 1 [ Mus musculus (house mouse) ]
• Official Symbol: Sphk1
• Synonyms: Sphk1; sphingosine kinase 1; SK; Sk1; Spk1; 1110006G24Rik; sphingosine kinase 1; acetyltransferase SPHK1; EC 2.7.1.91
• Gene ID: 20698
• mRNA Refseq: NM_011451
• Protein Refseq: NP_035581
• UniProt ID: Q8CI15

Reviews

04/16/2023

Its seamless integration makes it possible to explore different research avenues, expanding the scope and impact of my investigations.

04/14/2020

With the manufacturer's excellent technical support and its wide range of applications, the SPHK1 protein has become an indispensable tool, aiding in my research endeavors and driving meaningful scientific discoveries.

01/03/2020

Their team of experts is readily available to address any issues that may arise, providing solutions to problems and guiding me through the experimental process.

Q&As

How does SPHK1 contribute to drug resistance in cancer?

Elevated SPHK1 levels have been associated with drug resistance in cancer cells, making it a potential target to overcome resistance mechanisms.

How does SPHK1 contribute to immune regulation?

SPHK1 regulates immune cell trafficking and function by modulating S1P levels. This is significant in autoimmune diseases and inflammatory responses.

How might the modulation of SPHK1 impact personalized medicine?

Understanding the role of SPHK1 in different diseases may contribute to the development of personalized therapeutic approaches, tailoring treatments based on individual patient characteristics and disease profiles.

How does SPHK1 influence vascular functions?

SPHK1 is involved in endothelial cell functions and angiogenesis, making it a potential target in the treatment of vascular diseases.

Can SPHK1 inhibitors be used in combination therapies?

Yes, combining SPHK1 inhibitors with other therapeutic agents is being explored to enhance treatment efficacy, especially in cancer.