"Sphk1" Related Products

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Recombinant Mouse Sphk1 protein, Met/His-tagged

Cat.No.: Sphk1-526M
Product Overview: Recombinant Mouse Sphk1(Glu2-Pro382) fused with an N-terminal Met and 6-His tag was expressed in Insect cells.
Source: Insect cells
Species: Mouse
Tag: His
Form: Supplied as a 0.2 μm filtered solution in MES, NaCl, Glycerol and DTT.
Bio-activity: Measured by its ability to phosphorylate Omega-(7-nitro-2-1,3-benzoxadiazol-4-yl)-D-erythro-sphingosine (NBD-sphingosine). The specific activity is >2,500 pmol/min/μg.
Molecular Mass: Predicted Molecular Mass: 43 kDa;SDS-PAGE: 42 kDa, reducing conditions;
Endotoxin: <1.0 EU per 1 μg of the protein by the LAL method.
Purity: >70%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane
Storage: Avoid repeated freeze-thaw cycles.6 months from date of receipt, -70 centigrade as supplied.3 months, -70 centigrade under sterile conditions after opening.
Gene Name: Sphk1 sphingosine kinase 1 [ Mus musculus ]
Official Symbol: Sphk1
Synonyms: SPHK1; sphingosine kinase 1; SK 1; SPK 1; Sk1; Spk1; 1110006G24Rik;
Gene ID: 20698
mRNA Refseq: NM_001172472
Protein Refseq: NP_001165943
MIM:
UniProt ID: Q8CI15
Chromosome Location: 11; 11 E2
Pathway: Association of TriC/CCT with target proteins during biosynthesis, organism-specific biosystem; Calcium signaling pathway, organism-specific biosystem; Calcium signaling pathway, conserved biosystem; Chaperonin-mediated protein folding, organism-specific biosystem; Fc gamma R-mediated phagocytosis, organism-specific biosystem; Fc gamma R-mediated phagocytosis, conserved biosystem; Metabolic pathways, organism-specific biosystem;
Function: ATP binding; D-erythro-sphingosine kinase activity; DNA binding; calmodulin binding; calmodulin binding; diacylglycerol kinase activity; kinase activity; magnesium ion binding; nucleotide binding; protein phosphatase 2A binding; sphinganine kinase activity; sphinganine kinase activity; transferase activity;

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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