Recombinant Mouse SPN cell lysate

Cat.No. : SPN-1739MCL
Product Overview : Mouse SPN / CD43 derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls
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Source : Human cells
Species : Mouse
Preparation method : Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis buffer : Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality control Testing : 12.5% SDS-PAGE Stained with Coomassie Blue
Recommended Usage : 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Stability : Samples are stable for up to twelve months from date of receipt at -80°C
Storage Buffer : 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Storage Instruction : Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Gene Name : Spn sialophorin [ Mus musculus ]
Official Symbol : SPN
Synonyms : SPN; sialophorin; leukosialin; 3E8 antigen; lymphocyte antigen 48; leukocyte sialoglycoprotein; B-cell differentiation antigen LP-3; Cd43; Ly48; Galgp; Ly-48; A630014B01Rik;
Gene ID : 20737
mRNA Refseq : NM_001037810
Protein Refseq : NP_001032899
Pathway : Basigin interactions, organism-specific biosystem; Cell adhesion molecules (CAMs), organism-specific biosystem; Cell adhesion molecules (CAMs), conserved biosystem; Cell surface interactions at the vascular wall, organism-specific biosystem; Hemostasis, organism-specific biosystem;
Function : bacterial cell surface binding; bacterial cell surface binding; protein binding; protein domain specific binding;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (7)

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What is the primary role of SPN in immune cell function? 12/29/2022

SPN, or Sialophorin, is important in modulating the function of immune cells, particularly lymphocytes.

What potential therapeutic applications arise from targeting SPN in immune disorders? 12/28/2022

Targeting SPN could provide new treatment approaches for managing immune-related conditions, including autoimmune diseases.

How do genetic variations in SPN affect immune response? 07/10/2019

Genetic changes in SPN can lead to variations in immune cell behavior and effectiveness.

What role does SPN play in cell adhesion and migration? 12/12/2018

SPN plays a role in regulating immune cell adhesion and migration, crucial for immune surveillance and response.

How does SPN interact with other components of the immune system? 05/13/2018

SPN interacts with other immune molecules, coordinating immune cell communication and function.

What is the impact of altered SPN activity on autoimmune diseases? 02/12/2018

Altered SPN activity can impact the regulation of immune responses, potentially leading to autoimmune disorders.

How does SPN contribute to immune cell signaling? 08/11/2017

It contributes to immune cell signaling, influencing cell activation and response to stimuli.

Customer Reviews (3)

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06/23/2023

    Accurate and timely results, crucial for research progress.

    10/02/2018

      Instrumental in our research achievements, highly trusted.

      03/26/2018

        Efficient and dependable, aids our research significantly.

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