Active Recombinant Mouse Tlr4 Protein, Fc-tagged

• Cat.No.: Tlr4-02M
• Product Overview: Recombinant Mouse TLR4 (Asn26-Lys629) protein with a mouse IgG2a (Glu98-Lys330) Fc tag was expressed in chinese hamster ovary cell line.

Specification

• Description: This gene belongs to the evolutionarily-conserved Toll-like receptor family, whose members are type-1 transmembrane proteins that are involved in innate immunity. Toll-like receptors are characterized by an extracellular leucine-rich repeat domain that functions in ligand recognition and an intracellular toll/interleukin-1 receptor-like domain that is crucial for signal transduction. The receptor encoded by this gene mediates the innate immune response to bacterial lipopolysaccharide, a major component of the outer membrane of Gram-negative bacteria, through synthesis of pro-inflammatory cytokines and chemokines. In addition, this protein can recognize other pathogens from Gram-negative and Gram-positive bacteria as well as viral components. Mice deficient in this gene display a number of immune response-related phenotypes including hyporesponsiveness to bacterial lipopolysaccharide and increased levels of respiratory syncytial virus compared to controls.
• Source: CHO
• Species: Mouse
• Tag: Fc
• Form: Disulfide-linked homodimer
• Bio-activity: Measured by its binding ability in a functional ELISA. When Recombinant Mouse TLR4 Fc Chimera is immobilized at 2 μg/mL, 100 μL/well, the concentration of Recombinant Human MD-2 that produces 50% of the optimal binding response is approximately 0.15-0.9 μg/mL
• Molecular Mass: 96 kDa
• Protein length: 26-629
• N-terminal Sequence Analysis: Asn26
• Endotoxin: < 0.10 EU/μg of the protein by the LAL method.
• Purity: > 90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 centigrade as supplied.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Reconstitution: Reconstitute at 500 μg/mL in PBS.
• Shipping: The product is shipped with polar packs.

Gene Information

• Gene Name: Tlr4 toll-like receptor 4 [ Mus musculus (house mouse) ]
• Official Symbol: Tlr4
• Synonyms: Tlr4; toll-like receptor 4; Lps; Ras; Ly87; Ran/M1; Rasl2-8; toll-like receptor 4; Toll like receptor 4; lipopolysaccharide response; EC 3.2.2.6
• Gene ID: 21898
• mRNA Refseq: NM_021297
• Protein Refseq: NP_067272
• UniProt ID: Q9QUK6