Recombinant Mouse Washc3 Protein, Myc/DDK-tagged

• Cat.No.: Washc3-6965M
• Product Overview: Purified recombinant protein of Mouse WASH complex subunit 3 (Washc3), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: Acts as a component of the WASH core complex that functions as a nucleation-promoting factor (NPF) at the surface of endosomes, where it recruits and activates the Arp2/3 complex to induce actin polymerization, playing a key role in the fission of tubules that serve as transport intermediates during endosome sortingg.
• Molecular Mass: 21.1 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Washc3 WASH complex subunit 3 [ Mus musculus (house mouse) ]
• Official Symbol: Washc3
• Synonyms: Washc3; WASH complex subunit 3; Ccdc53; 2900091E11Rik; 5730495F03Rik; WASH complex subunit 3; WASH complex subunit CCDC53; coiled-coil domain containing 53; coiled-coil domain-containing protein 53
• Gene ID: 67282
• mRNA Refseq: NM_026070
• Protein Refseq: NP_080346
• UniProt ID: Q9CR27