Active Recombinant Mouse Wnt-1/sFRP-1 Complex Protein

• Cat.No.: Wnt1-001M
• Product Overview: Recombinant mouse Wnt-1 (Ala28-Leu370) and sFRP-1 (Ser32-Lys314) complex protein without tag was expressed in Chinese hamster ovary cell.

Specification

• Description: Biased expression in CNS E11.5 (RPKM 2.1), testis adult (RPKM 1.8) and 5 other tissues.
• Source: CHO Cell
• Species: Mouse
• Bio-activity: Measured by its ability to activate Wnt induced TCF reporter activity in HEK293 human embryonic kidney cells. The ED50 for this effect is 0.03-0.18 μg/mL
• Molecular Mass: 38 kDa (Wnt-1) & 33 kDa (sFRP-1)
• Endotoxin: <1.0 EU per 1 μg of the protein by the LAL method.
• Purity: >85%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 centigrade as supplied.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution in MOPS, NaCl, Trehalose and with BSA as a carrier protein.
• Reconstitution: Reconstitute at 100 μg/mL in PBS.
• Shipping: The product is shipped at ambient temperature.

Gene Information

• Gene Name: Wnt1 wingless-type MMTV integration site family, member 1 [ Mus musculus (house mouse) ]
• Official Symbol: Wnt1
• Synonyms: Wnt1; wingless-type MMTV integration site family, member 1; sw; Int-1; Wnt-1; swaying; proto-oncogene Wnt-1; proto-oncogene Int-1; proto-oncogene protein Wnt-1; wingless-related MMTV integration site 1
• Gene ID: 22408
• mRNA Refseq: NM_021279
• Protein Refseq: NP_067254
• UniProt ID: P04426

Reviews

10/10/2020

The manufacturer's commitment to providing high-quality proteins is evident in their meticulous production process, ensuring the purity and reliability of the WNT1 protein.

10/25/2019

The WNT1 protein is characterized by its exceptional quality, making it an ideal choice to fulfill my experimental requirements.

05/07/2017

the WNT1 protein's high quality and its compatibility with my experimental needs, coupled with the manufacturer's excellent technical support, create a valuable alliance that contributes to successful research endeavors.

Q&As

Can WNT1 be used as a diagnostic marker for certain cancers?

Research suggests that elevated levels of WNT1 may serve as a diagnostic marker for certain cancers, aiding in early detection.

Are there therapeutic interventions targeting WNT1 for neurodegenerative diseases?

Some studies are exploring the modulation of WNT1 signaling as a potential therapeutic approach for neurodegenerative diseases.

Is WNT1 involved in bone formation and regeneration?

Yes, WNT1 plays a crucial role in bone development and regeneration, and its manipulation is being explored for potential applications in bone-related disorders.

Are there any ongoing clinical trials targeting WNT1 in cancer treatment?

Yes, some clinical trials are exploring the inhibition of WNT1 as a potential strategy for treating specific types of cancers.

How does WNT1 relate to neurodegenerative diseases?

WNT1 has been implicated in neurodegenerative diseases such as Alzheimer's and Parkinson's, where its dysregulation may contribute to the pathology.