Recombinant Mouse Wnt-1/sFRP-1 Complex Protein
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|Product Overview :||Recombinant mouse Wnt-1 (Ala28-Leu370) and sFRP-1 (Ser32-Lys314) complex protein without tag was expressed in Chinese hamster ovary cell.|
|Description :||Biased expression in CNS E11.5 (RPKM 2.1), testis adult (RPKM 1.8) and 5 other tissues.|
|Source :||CHO Cell|
|Bio-activity :||Measured by its ability to activate Wnt induced TCF reporter activity in HEK293 human embryonic kidney cells. The ED50 for this effect is 0.03-0.18 μg/mL|
|Molecular Mass :||38 kDa (Wnt-1) & 33 kDa (sFRP-1)|
|Endotoxin :||<1.0 EU per 1 μg of the protein by the LAL method.|
|Purity :||>85%, by SDS-PAGE under reducing conditions and visualized by silver stain.|
|Storage :||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
|Storage Buffer :||Lyophilized from a 0.2 μm filtered solution in MOPS, NaCl, Trehalose and with BSA as a carrier protein.|
|Reconstitution :||Reconstitute at 100 μg/mL in PBS.|
|Shipping :||The product is shipped at ambient temperature.|
|Gene Name :||Wnt1 wingless-type MMTV integration site family, member 1 [ Mus musculus (house mouse) ]|
|Official Symbol :||Wnt1|
|Synonyms :||Wnt1; wingless-type MMTV integration site family, member 1; sw; Int-1; Wnt-1; swaying; proto-oncogene Wnt-1; proto-oncogene Int-1; proto-oncogene protein Wnt-1; wingless-related MMTV integration site 1|
|Gene ID :||22408|
|mRNA Refseq :||NM_021279|
|Protein Refseq :||NP_067254|
|UniProt ID :||P04426|
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