Active Recombinant Pig MMP9 protein, His-tagged
Cat.No. : | MMP9-1131P |
Product Overview : | Active Recombinant Pig MMP9 protein(Q2VI03)(169~387 aa), fused with N-terminal His tag, was expressed in E.coli. |
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Species : | Pig |
Source : | E.coli |
Tag : | His |
Protein Length : | 169-387 aa |
Form : | Lyophilized from sterile 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300. |
Bio-activity : | MMP9 is a zinc-dependent enzymes capable of cleaving components of the extracellular matrix, which belongs to the matrix metalloproteinase (MMP) family. It is a gelatinase A, 92kDa type IV collagenase which can hydrolyze gelatin under certain conditions. Gelatin zymography is mainly used for the detection of the gelatinases, MMP-2 and MMP-9 and It is extremely sensitive because levels of 10pg of MMP-2 can already be detected. Briefly, various concentrations of recombinant pig MMP9 (1000ng, 500ng, 100ng, 10ng, 1ng, 0.1ng) were denatured by SDS loading buffer, electrophoresed through sodium dodecylsulphate- polyacrylamide gel (SDS–PAGE; 10% gels) containing gelatin (1mg/mL) with nonreducing conditions. After renaturation, incubation and CCB-stained, active MMP9 would hydrolyze gelatin nearby, which was indicated by the white binds on the gel. In this experiment we use heat-denatured MMP9 protein as negative control, and blood sample as positive control. |
Molecular Mass : | 27.9kDa |
Endotoxin : | <1.0EU per 1μg (determined by the LAL method). |
Purity : | > 90% as determined by SDS-PAGE. |
Storage : | Avoid repeated freeze/thaw cycles. Store at -20°C for 12 months. Aliquot and store at -80°C for 12 months. |
Reconstitution : | Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex. |
Gene Name | MMP9 matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase) [ Sus scrofa ] |
Official Symbol | MMP9 |
Synonyms | MMP9; matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase); matrix metalloproteinase-9; matrix metalloproteinase 9; |
Gene ID | 654325 |
mRNA Refseq | NM_001038004 |
Protein Refseq | NP_001033093 |
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Not For Human Consumption!
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