Active Recombinant Rat CD274 Protein, His-tagged

• Cat.No.: CD274-11141R
• Product Overview: Active Recombinant Rat CD274 Protein(NP_001178883.1)(Ala18-Thr238) is expressed from HEK293 with His tag at the C-Terminus.

Specification

• Species: Rat
• Source: HEK293
• Tag: His
• Protein Length: 18-238 aa
• Form: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized Rat PD-L1 at 10 μg/mL (100 μL/well) can bind Biotinylated Rat PD-1 with a linear range of 0.0195-2.16 μg/mL.
• Molecular Mass: 25.90 kDa
• Endotoxin: < 0.1 EU/μg of the protein by LAL method.
• Purity: ≥ 95 % as determined by SDS-PAGE.
• Storage: Store at -20°C.Store the lyophilized protein at -20°C to -80°C up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.
• Reconstitution: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

Gene Information

• Gene Name: Cd274 CD274 molecule [ Rattus norvegicus ]
• Official Symbol: CD274
• Synonyms: CD274; CD274 molecule; programmed cell death 1 ligand 1; Pdcd1lg1; RGD1566211
• Gene ID: 499342
• mRNA Refseq: NM_001191954
• Protein Refseq: NP_001178883