Recombinant Rat Car12 protein, His-tagged
Cat.No. : | Car12-7848R |
Product Overview : | Recombinant Rat Car12 aa. (Leu154~Ser307 (Accession # A2IBE2)) fused with N-terminal His tag was produced in E. coli cells. |
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Source : | E. coli |
Species : | Rat |
Tag : | His |
Form : | Freeze-dried powder |
Molecular Mass : | 22kDa as determined by SDS-PAGE reducing conditions. |
Protein length : | Leu154~Ser307 (Accession # A2IBE2) |
Endotoxin : | <1.0EU per 1ug (determined by the LAL method) |
Purity : | >95% |
Characteristic : | The isoelectric point is 5.5. |
Applications : | SDS-PAGE; WB; ELISA; IP |
Stability : | The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition. |
Storage : | Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months. |
Storage Buffer : | Supplied as lyophilized form in PBS, pH 7.4, containing 5% sucrose, 0.01% sarcosyl. |
Reconstitution : | Reconstitute in sterile PBS, pH7.2-pH7.4. |
Gene Name : | Car12 carbonic anhydrase 12 [ Rattus norvegicus (Norway rat) ] |
Official Symbol : | Car12 |
Synonyms : | Car12; carbonic anhydrase 12; carbonic anyhydrase 12; membrane-bound carbonic anhydrase 12 |
Gene ID : | 363085 |
mRNA Refseq : | NM_001080756.1 |
Protein Refseq : | NP_001074225.1 |
UniProt ID : | A2IBE2 |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Write a reviewProfessional assistance, invaluable for complex protein experiments.
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Q&As (7)
Ask a questionKnown inhibitors can be identified through drug screening assays, and their impact on CA12 can be studied using kinetic studies and cell-based assays.
Cellular localization can be identified using immunofluorescence, subcellular fractionation, or electron microscopy.
CA12 has been implicated in tumor progression, particularly in hypoxic environments, by modulating pH and thus influencing tumor cell survival and metastasis. This can be assessed using cellular assays and tumor models.
Downstream pathways can be identified using pathway analysis tools, phosphoproteomics, and specific signal transduction assays.
The activity modulation under different pH conditions can be determined using enzymatic assays and pH shift experiments.
Expression changes in response to stressors can be analyzed using techniques like qPCR, Western blotting, or proteomics following cellular treatment with specific agents.
Genetic variants and their impact can be determined using structural models and functional assays comparing wild-type and mutant proteins.
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