Recombinant Rat Car3 protein, His & T7-tagged

• Cat.No.: Car3-7834R
• Product Overview: Recombinant Rat Car3 aa. (Asn27~Val217 (Accession # P14141)) fused with N-terminal His & T7 tag was produced in E. coli cells.

Specification

• Description: Catalyzes hydration of carbon dioxide; may be involved in cellular response to oxidative stress.
• Source: E. coli
• Species: Rat
• Tag: His&T7
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 25.5kDa
• Protein length: Asn27~Val217 (Accession # P14141)
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 6.8.
• Applications: SDS-PAGE; WB; ELISA; IP
• Stability: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage Buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: Car3 carbonic anhydrase 3 [ Rattus norvegicus (Norway rat) ]
• Official Symbol: Car3
• Synonyms: Car3; carbonic anhydrase 3; Ca3; CA-III; carbonate dehydratase III; carbonic anhydrase III
• Gene ID: 54232
• mRNA Refseq: NM_019292.4
• Protein Refseq: NP_062165.2
• UniProt ID: P14141

Reviews

09/23/2022

Outstanding protein sequencing capabilities, unrivaled accuracy.

05/23/2022

Precise purification techniques, yield exceptional samples.

05/25/2021

Efficient sample handling, optimizes our laboratory workflow.

Q&As

In which tissues is CA3 expression most prominently observed?

CA3 is primarily found in skeletal muscles.

What post-translational modifications are characteristic of CA3?

Post-translational modifications, like phosphorylation or glycosylation, can be identified using mass spectrometry.

Are there known inhibitors that specifically target CA3?

Specific inhibitors for CA3 are yet to be fully elucidated and would be determined using enzymatic assays.

How does CA3 participate in metabolic processes within the cell?

CA3 plays a role in CO2 and bicarbonate equilibrium, crucial for metabolic pathways like glycolysis and the citric acid cycle.

How does CA3 enzymatic activity differ from other carbonic anhydrase isoforms?

While CA3 has a similar mechanism, its activity and affinity might differ from other isoforms, requiring comparative studies.

Are there any pathological conditions directly linked to CA3 dysregulation?

CA3 dysregulation might be linked to muscle diseases or metabolic disorders, requiring further research.

What is the primary physiological role of CA3 in cells?

CA3 catalyzes the reversible hydration of carbon dioxide, aiding in pH regulation and CO2 transport.