Recombinant Rat Casp9 protein, His-tagged

• Cat.No.: Casp9-629R
• Product Overview: Recombinant Rat Casp9 aa. (Met1~His200) fused with N-terminal His tag was produced in E. coli cells.

Specification

• Description: This gene is part of a family of caspases, aspartate-specific cysteine proteases well studied for their involvement in immune and apoptosis signaling. This protein, the initiator caspase, is activated after cytochrome c release from mitochondria and targets downstream effectors. In mouse, deficiency of this gene can cause perinatal lethality. This protein may have a role in normal brain development. Alternative splicing results in multiple transcript variants that encode different protein isoforms.
• Source: E. coli
• Species: Rat
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: 26kDa as determined by SDS-PAGE reducing conditions
• Protein length: Met1~His200
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: >92%
• Characteristic: The isoelectric point is 6.1.
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex

Gene Information

• Gene Name: Casp9 caspase 9 [ Mus musculus (house mouse) ]
• Official Symbol: Casp9
• Synonyms: Casp9; caspase 9; Mch6; APAF-3; CASP-9; AI115399; AW493809; ICE-LAP6; Caspase-9; ICE-like apoptotic protease 6; apoptotic protease Mch-6; apoptotic protease-activating factor 3
• Gene ID: 12371
• mRNA Refseq: NM_001277932.1
• Protein Refseq: NP_001264861.1
• UniProt ID: A2AS93

Reviews

04/12/2023

Its purity and consistency have provided me with reliable and reproducible results, ensuring the accuracy of my research findings.

04/27/2020

Their expertise and guidance have helped overcome hurdles, ensuring the smooth progress of my research.

10/27/2016

The bands of proteins in Western Blotting experiments were very clear.

Q&As

What clinical conditions are associated with dysregulation of CASP9 protein?

Dysregulation of CASP9 protein is associated with cancer, neurodegenerative diseases, and immune system disorders.

Can CASP9 protein be used as a therapeutic target in autoimmune diseases?

CASP9 protein is being investigated as a potential therapeutic target in autoimmune diseases to modulate immune cell apoptosis.

How is CASP9 protein implicated in immune system disorders?

Dysregulation of CASP9 protein can lead to abnormal immune cell apoptosis, contributing to autoimmune diseases and immunodeficiency.

How is CASP9 protein targeted in cancer therapy?

CASP9 protein is targeted using small molecule inhibitors and therapeutic antibodies to induce apoptosis in cancer cells.

Are there any genetic variations in the CASP9 gene that affect protein function?

Yes, genetic variations in the CASP9 gene can impact protein function and have been linked to disease susceptibility.