Recombinant Rat Ccl7 protein
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Cat.No. : | Ccl7-635R Optional Service: Optional requirements on this protein |
Product Overview : | Recombinant Rat Ccl7 protein was expressed in Escherichia coli. |
Description : | Rat CCL7, belonging to the CC chemokine family, is also named as MCP-3, because the sequence comparison suggested that the rat CCL7 is a homologue of the human MCP-3 and is postulated the rat MCP-3. The MCP-3 protein family signals through CCR2 expect MCP-1 and possess cross-reacts across species. CCL7/MCP3 has chemotactic functions for monocytes and eosinophils, but not for neutrophils. Additionally, it can activated NK cells as well as CD4+ and CD8+ T lymphocytes. CCL7 was found to interact with MMP2. |
Source : | E.coli |
Species : | Rat |
Form : | Lyophilized from a 0.2μm filtered concentrated solution in 2 × PBS, pH 7.4. |
Bio-activity : | Fully biologically active when compared to standard. The biologically active determined by a chemotaxis bioassay using human monocytes is in a concentration range of 10-100 ng/ml. |
Molecular Mass : | Approximately 8.5 kDa, a single non-glycosylated polypeptide chain containing 74 amino acids. |
Protein length : | 74 |
AA Sequence : | QPDGTNSSTC CYVKKQKIPK RNLKSYRKIT SSRCPWEAVI FKTKKGMEVC AEAHQKWVEE AIAYLDMKTS TPKP |
Endotoxin : | Less than 1 EU/µg of rRtMCP-3/CCL7 as determined by LAL method. |
Purity : | >95% by SDS-PAGE and HPLC analysis. |
Storage : | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, -20 to -70 centigrade under sterile conditions after reconstitution. |
Reconstitution : | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤-20 centigrade. Further dilutions should be made in appropriate buffered solutions. |
SDS-PAGE of Ccl7-635R : |
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Gene Name : | Ccl7 |
Official Symbol : | Ccl7 |
Synonyms : | CCL7; chemokine (C-C motif) ligand 7; C-C motif chemokine 7; MCP-3; chemotactic protein-3; small-inducible cytokine A7; monocyte chemotactic protein 3; monocyte chemoattractant protein 3; |
Gene ID : | 287561 |
mRNA Refseq : | NM_001007612 |
Protein Refseq : | NP_001007613 |
UniProt ID : | Q9QXY8 |
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◆ Lysates
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CCL1CCL11Ccl12CCL13CCL14CCL15CCL16CCL17CCL18CCL19CCL2CCL20CCL21Ccl21cCCL22CCL23CCL24CCL25CCL26CCL27Ccl27aCCL28CCL3CCL3L1Ccl4CCL5Ccl6CCL7CCL8Ccl9CCL4L2Ccl21aCCL4L1CCL3L3Ccl21bCcl27b
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