Recombinant Rat CD4 Protein, His-tagged

Cat.No.: CD4-1075R
Product Overview: Recombinant rat Cd4 (NP_036837.1) (Met1-Thr394) was expressed with a polyhistidine tag at the C-terminus.
Source: HEK293
Species: Rat
Tag: His
Predicted N Terminal: Lys 28
Form: Lyophilized from sterile PBS, pH 7.4, 5%~8% trehalose and mannitol.
Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of NIH-3T3 mouse embryonic fibroblast cells.When 5 × 10^4 cells/well areadded to ratCD4-His coated plates (0.2μg/mL and 100μL/well), approximately >70% will adhere specifically aft
Molecular Mass: The recombinant rat Cd4 consists 378 amino acids and predicts a molecular mass of 42.5 kDa.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Purity: >95 % as determined by SDS-PAGE.
Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
Gene Name: Cd4 Cd4 molecule [ Rattus norvegicus ]
Official Symbol: CD4
Synonyms: CD4; Cd4 molecule; T-cell surface glycoprotein CD4; CD4 antigen; W3/25 antigen; T-cell surface antigen T4/Leu-3; p55; W3/25;
Gene ID: 24932
mRNA Refseq: NM_012705
Protein Refseq: NP_036837
MIM:
UniProt ID:
Pathway: Adaptive Immune System, organism-specific biosystem; Antigen processing and presentation, organism-specific biosystem; Antigen processing and presentation, conserved biosystem; Cell adhesion molecules (CAMs), organism-specific biosystem; Cell adhesion mol
Function: enzyme binding; enzyme binding; glycoprotein binding; glycoprotein binding; protein homodimerization activity; protein homodimerization activity; protein kinase binding; protein kinase binding; zinc ion binding; zinc ion binding;

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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