Recombinant Rat DUSP6 Protein

• Cat.No.: DUSP6-1978R
• Product Overview: Recombinant Rat DUSP6 full length or partial length protein was expressed.

Specification

• Species: Rat
• Source: Mammalian Cells
• Tag: His
• Form: Liquid or lyophilized powder
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: >80%
• Notes: This item requires custom production and lead time is between 5-9 weeks. We can custom produce according to your specifications.
• Storage: Store it at +4 ºC for short term. For long term storage, store it at -20 ºC~-80 ºC.
• Storage Buffer: PBS buffer

Gene Information

• Gene Name: Dusp6 dual specificity phosphatase 6 [ Rattus norvegicus ]
• Official Symbol: DUSP6
• Gene ID: 116663
• mRNA Refseq: NM_053883.2
• Protein Refseq: NP_446335.1
• UniProt ID: Q64346

Citation

Excess PLAC8 promotes an unconventional ERK2-dependent EMT in colon cancer

Journal: The Journal of Clinical Investigation    PubMed ID: 24691442    Data: 2014/5/1

Authors: Cunxi Li, Haiting Ma, Robert J. Coffey

Article Snippet:Recombinant full-length DUSP6 and DUSP3 proteins were purchased from Creative BioMart.. Phosphatase activity was measured using 0.1 mg/ml 3-O-methylfluorescein phosphate as the substrate in a 96-well plate assay with a BioTek Synergy 4 plate reader (excitation 485 nm, emission 528 nm) ( 88 , 89 ).Phosphatase activity was measured using 0.1 mg/ml 3-O-methylfluorescein phosphate as the substrate in a 96-well plate assay with a BioTek Synergy 4 plate reader (excitation 485 nm, emission 528 nm) ( 88 , 89 ).

(A) PLAC8 inhibited DUSP6 phosphatase activity in vitro. DUSP6 phosphatase activity was measured by fluorescent intensity of the substrate 3-O-methylfluorescein phosphate. Fluorescent intensity increased over time in control samples (black squares). Addition of purified MBP-tagged mouse PLAC8 protein significantly reduced DUSP6 phosphatase activity (blue circles). However, addition of MBP itself did not significantly affect the activity. Phosphatase inhibitor cocktail was added as a positive control to completely abolish the activity (red squares). Data are presented as mean ± SEM from 4 independent experiments. *P < 0.05, ANOVA followed by t test. (B) Coimmunoprecipitation shows interaction between PLAC8 and DUSP6. HEK293T cells were transfected with the plasmids as labeled. PLAC8 was coimmunoprecipitated with an anti-Myc antibody from cells expressing Myc-tagged DUSP6, but not from cells expressing only PLAC8.