Recombinant Rat DUSP6 Protein

Cat.No. : DUSP6-1978R
Product Overview : Recombinant Rat DUSP6 full length or partial length protein was expressed.
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Species : Rat
Source : Mammalian Cells
Tag : His
Form : Liquid or lyophilized powder
Endotoxin : < 1.0 EU per μg of the protein as determined by the LAL method.
Purity : >80%
Notes : This item requires custom production and lead time is between 5-9 weeks. We can custom produce according to your specifications.
Storage : Store it at +4 ºC for short term. For long term storage, store it at -20 ºC~-80 ºC.
Storage Buffer : PBS buffer
Gene Name Dusp6 dual specificity phosphatase 6 [ Rattus norvegicus ]
Official Symbol DUSP6
Gene ID 116663
mRNA Refseq NM_053883.2
Protein Refseq NP_446335.1
MIM
UniProt ID Q64346

Excess PLAC8 promotes an unconventional ERK2-dependent EMT in colon cancer

Journal: The Journal of Clinical Investigation    PubMed ID: 24691442    Data: 2014/5/1

Authors: Cunxi Li, Haiting Ma, Robert J. Coffey

Article Snippet:Recombinant full-length DUSP6 and DUSP3 proteins were purchased from Creative BioMart.. Phosphatase activity was measured using 0.1 mg/ml 3-O-methylfluorescein phosphate as the substrate in a 96-well plate assay with a BioTek Synergy 4 plate reader (excitation 485 nm, emission 528 nm) ( 88 , 89 ).Phosphatase activity was measured using 0.1 mg/ml 3-O-methylfluorescein phosphate as the substrate in a 96-well plate assay with a BioTek Synergy 4 plate reader (excitation 485 nm, emission 528 nm) ( 88 , 89 ).

(A) PLAC8 inhibited DUSP6 phosphatase activity in vitro. DUSP6 phosphatase activity was measured by fluorescent intensity of the substrate 3-O-methylfluorescein phosphate. Fluorescent intensity increased over time in control samples (black squares). Addition of purified MBP-tagged mouse PLAC8 protein significantly reduced DUSP6 phosphatase activity (blue circles). However, addition of MBP itself did not significantly affect the activity. Phosphatase inhibitor cocktail was added as a positive control to completely abolish the activity (red squares). Data are presented as mean ± SEM from 4 independent experiments. *P < 0.05, ANOVA followed by t test. (B) Coimmunoprecipitation shows interaction between PLAC8 and DUSP6. HEK293T cells were transfected with the plasmids as labeled. PLAC8 was coimmunoprecipitated with an anti-Myc antibody from cells expressing Myc-tagged DUSP6, but not from cells expressing only PLAC8.

(A) PLAC8 inhibited DUSP6 phosphatase activity in vitro. DUSP6 phosphatase activity was measured by fluorescent intensity of the substrate 3-O-methylfluorescein phosphate. Fluorescent intensity increased over time in control samples (black squares). Addition of purified MBP-tagged mouse PLAC8 protein significantly reduced DUSP6 phosphatase activity (blue circles). However, addition of MBP itself did not significantly affect the activity. Phosphatase inhibitor cocktail was added as a positive control to completely abolish the activity (red squares). Data are presented as mean ± SEM from 4 independent experiments. *P < 0.05, ANOVA followed by t test. (B) Coimmunoprecipitation shows interaction between PLAC8 and DUSP6. HEK293T cells were transfected with the plasmids as labeled. PLAC8 was coimmunoprecipitated with an anti-Myc antibody from cells expressing Myc-tagged DUSP6, but not from cells expressing only PLAC8.

Not For Human Consumption!

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