Recombinant Rat F11 protein, His & GST-tagged

• Cat.No.: F11-1862R
• Product Overview: Recombinant Rat F11 aa. (Ile275~Gly516) fused with N-terminal His & GST tag was produced in E. coli cells.

Specification

• Source: E. coli
• Species: Rat
• Tag: His & GST
• Form: Freeze-dried powder
• Molecular Mass: 57kDa as determined by SDS-PAGE reducing conditions.
• Protein length: Ile275~Gly516
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >98%
• Characteristic: The isoelectric point is 6.5.
• Applications: SDS-PAGE; WB; ELISA; IP; CoIP; Purification; Amine Reactive Labeling.
• Stability: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Concentration: 200μg/mL
• Storage Buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0mg/mL. Do not vortex.

Gene Information

• Gene Name: F11 coagulation factor XI [ Rattus norvegicus (Norway rat) ]
• Official Symbol: F11
• Synonyms: CYP1A1; cytochrome P450 family 1 subfamily A member 1; AHH; AHRR; CP11; CYP1; CYPIA1; P1-450; P450-C; P450DX; cytochrome P450 1A1; aryl hydrocarbon hydroxylase; cytochrome P1-450, dioxin-inducible; cytochrome P450 form 6; cytochrome P450, family 1, subfamily A, polypeptide 1; cytochrome P450, subfamily I (aromatic compound-inducible), polypeptide 1; cytochrome P450-C; cytochrome P450-P1; flavoprotein-linked monooxygenase; xenobiotic monooxygenase
• Gene ID: 290757
• mRNA Refseq: NM_001047848.1
• Protein Refseq: NP_001041313.1
• UniProt ID: Q6TUF8

Reviews

11/12/2021

The structure of the recombinant protein was analyzed by nuclear magnetic resonance (NMR), X-ray crystallography or electron microscopy, and the results showed that the protein was stable.

05/24/2021

In the process of purification, the removal of impurities is good.

05/17/2021

There are no structural or functional defects.

Q&As

Does F11 play a role in diabetes?

F11 plays an important role in diabetes, and abnormal levels may lead to insulin resistance and lipid metabolism disorders, which can lead to diabetes.

What are the mutation types of F11?

The mutation types of F11 include point mutations, insertions/deletions, duplications, etc., which may cause structural and functional abnormalities of the protein.

How to study the regulatory mechanism of F11?

Studying the regulatory mechanism of F11 requires the comprehensive use of various experimental methods and techniques, such as gene knockout, transcriptome analysis, protein interaction, etc.

What disease might abnormal F11 levels suggest?

Abnormal F11 levels may indicate some diseases related to lipid metabolism, such as diabetes, obesity, hyperlipidemia, etc.

How can the disease be treated by regulating F11 levels?

There are no drugs that directly regulate F11 levels, but they can be affected by lifestyle adjustments such as diet and exercise.

What is the relationship between F11 and other genes or proteins?

F11 has a complex relationship with other genes or proteins, and can interact with other genes or proteins and participate in a variety of biochemical reactions.