Recombinant Rat IGF1R protein, His-tagged
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|Product Overview :||Recombinant Rat IGF1R protein (Glu31-His936), fused to His tag at C-terminus, was expressed in human 293 cells (HEK293).|
|Description :||The Insulin-like Growth Factor 1 Receptor (IGF1) is also known as CD221, JTK13. and is a transmembrane receptor that is activated by IGF-1 and by the related growth factor IGF-2. It belongs to the large class of tyrosine kinase receptors. This receptor mediates the effects of IGF-1, which is a polypeptide protein hormone similar in molecular structure to insulin. IGF1R is make up of two alpha subunits and two beta subunits ,the Both the α and β subunits are synthesized from a single mRNA precursor. The precursor is then glycosylated, proteolytically cleaved, and crosslinked by cysteine bonds to form a functional transmembrane αβ chain.The α chains are located extracellularly while the β subunit spans the membrane and are responsible for intracellular signal transduction upon ligand stimulation. IGF1R have a binding site for ATP, which is used to provide the phosphates for autophosphorylation. There is a 60% homology between IGF1R and the insulin receptor. In response to ligand binding, the α chains induce the tyrosine autophosphorylation of the β chains. This event triggers a cascade of intracellular signaling that, while somewhat cell type specific, often promotes cell survival and cell proliferation.|
|Form :||Lyophilized from 0.22 um filtered solution in PBS, pH7.4, 10% trehalose.|
|Molecular Mass :||The protein has a calculated MW of 105.5 kDa, 81.3 kDa, 24.2 kDa. The protein migrates as 45-50 kDa, 96-115 kDa and 120 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.|
|Protein length :||906|
|Endotoxin :||Less than 1.0 EU per ug by the LAL method.|
|Purity :||>90% as determined by SDS-PAGE.|
For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20 centigrade to -70 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
|Reconstitution :||It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.|
|SDS-PAGE of IGF1R-393R :||
Rat IGF-I R on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
|Bioactivity-ELISA of IGF1R-393R :||
Immobilized Rat IGF-IR, His Tag at 5 μg/mL (100 μL/well) can bind Human IGF-I, Fc Tag with a linear range of 0.01-0.313 μg/mL (QC tested).
|Gene Name :||IGF1R|
|Official Symbol :||IGF1R|
|Synonyms :||IGF1R; insulin-like growth factor 1 receptor; IGF-I receptor; insulin-like growth factor I receptor; JTK13; IGFIRC|
|Gene ID :||25718|
|mRNA Refseq :||NM_052807|
|Protein Refseq :||NP_434694|
|UniProt ID :||P24062|
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◆ Assay kits
|Kit-1601||HEK 293 IGF1R Bioassay Kit||+Inquiry|
|Kit-1600||IGF1R Functional Assay Kit||+Inquiry|