Recombinant Rat IGF1R protein, His-tagged IGF1R-393R

Recombinant Rat IGF1R protein, His-tagged

PRODUCTS

Home / Products / Recombinant Proteins / Recombinant Rat IGF1R protein, His-tagged

Recombinant Rat IGF1R protein, His-tagged

IGF1R Related Products

Price Inquiry

Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.

Cat.No. : IGF1R-393R
Product Overview : Recombinant Rat IGF1R protein (Glu31-His936), fused to His tag at C-terminus, was expressed in human 293 cells (HEK293).
Description : The Insulin-like Growth Factor 1 Receptor (IGF1) is also known as CD221, JTK13. and is a transmembrane receptor that is activated by IGF-1 and by the related growth factor IGF-2. It belongs to the large class of tyrosine kinase receptors. This receptor mediates the effects of IGF-1, which is a polypeptide protein hormone similar in molecular structure to insulin. IGF1R is make up of two alpha subunits and two beta subunits ,the Both the α and β subunits are synthesized from a single mRNA precursor. The precursor is then glycosylated, proteolytically cleaved, and crosslinked by cysteine bonds to form a functional transmembrane αβ chain.The α chains are located extracellularly while the β subunit spans the membrane and are responsible for intracellular signal transduction upon ligand stimulation. IGF1R have a binding site for ATP, which is used to provide the phosphates for autophosphorylation. There is a 60% homology between IGF1R and the insulin receptor. In response to ligand binding, the α chains induce the tyrosine autophosphorylation of the β chains. This event triggers a cascade of intracellular signaling that, while somewhat cell type specific, often promotes cell survival and cell proliferation.
Source : HEK293
Species : Rat
Tag : His
Form : Lyophilized from 0.22 um filtered solution in PBS, pH7.4, 10% trehalose.
Molecular Mass : The protein has a calculated MW of 105.5 kDa, 81.3 kDa, 24.2 kDa. The protein migrates as 45-50 kDa, 96-115 kDa and 120 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Protein length : 906
Endotoxin : Less than 1.0 EU per ug by the LAL method.
Purity : >90% as determined by SDS-PAGE.
Storage : For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20 centigrade to -70 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
SDS-PAGE of IGF1R-393R :

Rat IGF-I R on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-ELISA of IGF1R-393R :

Immobilized Rat IGF-IR, His Tag at 5 μg/mL (100 μL/well) can bind Human IGF-I, Fc Tag with a linear range of 0.01-0.313 μg/mL (QC tested).

Gene Name : IGF1R
Official Symbol : IGF1R
Synonyms : IGF1R; insulin-like growth factor 1 receptor; IGF-I receptor; insulin-like growth factor I receptor; JTK13; IGFIRC
Gene ID : 25718
mRNA Refseq : NM_052807
Protein Refseq : NP_434694
UniProt ID : P24062

Online Inquiry


  • Note: There will be extra charge for optional service!
  • Please input "biomart" as verification code. Please review Creative BioMart's privacy policy for more information

Optional Service: Optional requirements on this protein

Other Requirements:

Apply For A Coupon

$50 OFF Your First Purchase

Apply For a Coupon

Enter your email here to subscribe.

creative biomart inc.

Easy access to products and services you need from our library via powerful searching tools.

Follow Us

Copyright © 2007 – 2020 Creative BioMart. All Rights Reserved. Terms and Conditions | Privacy Policy