Active Recombinant Rat Il2 protein, His-tagged

Cat.No. : Il2-480R
Product Overview : Active Recombinant Rat Il2 protein(P17108)(21~155 aa), fused with N-terminal His tag, was expressed in HEK293.
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Species : Rat
Source : HEK293
Tag : His
Protein Length : 21-155 aa
Form : Lyophilized from sterile PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
Bio-activity : IL-2(Interleukin-2) is a cytokine produced by T-cells in response to antigenic or mitogenic stimulation. IL-2 is a type of signaling molecule in the immune system, that is required for both T-cell and B-cell proliferation and other activities crucial to regulation of the immune response.Therefore, in order to detect the bioactivity of recombinant rat IL-2, spleen single suspensions were prepared, activated with conA (final concentration 3 ug/ml). Cells were collected after 72h and washed with hanks.Then mouse splenic lymphocytes were were seeded into triplicate wells of 96-well plates at a density of 10,000 cells/well with or without the addition of various concentrations of recombinant rat IL-2. After incubated for 72h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8(CCK-8). 10 µl of CCK-8 solution was added to each well of the plate, the absorbance at 450 nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37°C . Proliferation of Splenic lymphocytes cells after incubation with IL-2 for 72h observed by inverted microscope was shown.
Molecular Mass : 17.1kDa
Endotoxin : <1.0EU per 1μg (determined by the LAL method).
Purity : > 90% as determined by SDS-PAGE.
Storage : Avoid repeated freeze/thaw cycles. Store at -20°C for 12 months. Aliquot and store at -80°C for 12 months.
Reconstitution : Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Gene Name Il2 interleukin 2 [ Rattus norvegicus ]
Official Symbol Il2
Synonyms IL2; interleukin 2; interleukin-2; IL-2; TCGF; T-cell growth factor;
Gene ID 116562
mRNA Refseq NM_053836
Protein Refseq NP_446288

Not For Human Consumption!

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