Active Recombinant Rat Il21 protein, His-tagged

• Cat.No.: IL21-3039R
• Product Overview: Recombinant Rat Il21 protein(His18-Ser146), fused with C-terminal His tag, was expressed in HEK293.

Specification

• Species: Rat
• Source: HEK293
• Tag: His
• Protein Length: 18-146 aa
• Form: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
• Bio-activity: Measured by its ability to induce Interferon gamma secretion by human natural killer lymphoma NK-92 cells. The ED50 for this effect is 0.45-1.8 ng/mL, corresponding to a specific activity of 5.6×10^5~2.2×10^6 units/mg.
• Molecular Mass: 16.03 kDa
• Endotoxin: < 0.1 EU/μg of the protein by LAL method.
• Purity: ≥ 90% as determined by SDS-PAGE.
• Storage: Store at -20°C.Store the lyophilized protein at -20°C to -80°C up to 1 year from the date of receipt.After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.
• Reconstitution: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

Gene Information

• Gene Name: Il21 interleukin 21 [ Rattus norvegicus ]
• Official Symbol: Il21
• Synonyms: IL21; interleukin 21; interleukin-21; IL-21
• Gene ID: 365769
• mRNA Refseq: NM_001108943
• Protein Refseq: NP_001102413