Recombinant Rat NF1 Protein Pre-coupled Magnetic Beads

• Cat.No.: NF1-3625R-B
• Product Overview: The Recombnant protein was conjugated to magnetic beads. This ready-to-use, pre-coupled magnetic beads are in uniform particle size and narrow size distribution with large surface area, which is conducive to convenient and fast capture target molecules with high specificity and achieve magnetic separation. This product can be equipped with automation equipment for high-throughput operations.

Specification

• Source: HEK293
• Species: Rat
• Form: Solution
• Particle size: ~2 μm
• Beads Surface: Hydrophilic
• Capacity: > 200 pmol rabbit IgG/ mg beads
• Applications: Immunoassay, In vitro diagnostics, cell sorting, Immunoprecipitation/Co-precipitation, Protein/antibody separation and purification.
• Stability: Stable for at least 6 months from the date of receipt of the product under proper storage and handling conditions.
• Storage: 2-8℃. Do not to freeze thaw the Beads
• Concentration: 10mg beads/mL
• Storage Buffer: PBS buffer

Gene Information

• Gene Name: Nf1 neurofibromin 1 [ Rattus norvegicus ]
• Official Symbol: NF1
• Gene ID: 24592
• mRNA Refseq: NM_012609.1
• Protein Refseq: NP_036741.1
• UniProt ID: P97526

Reviews

07/08/2021

Clear instructions, easy protocol.

03/15/2021

Packaging was secure, intact.

06/27/2020

Product arrived in good condition.

Q&As

What is the primary function of the NF1 gene and its encoded protein?

The NF1 gene encodes neurofibromin, a protein primarily known for its role as a tumor suppressor. It regulates cell growth and differentiation.

How do mutations in the NF1 gene contribute to tumorigenesis?

Mutations in the NF1 gene can lead to a loss of neurofibromin function, resulting in uncontrolled RAS activation and increased cell proliferation, contributing to tumorigenesis.

How is the NF1 protein involved in the RAS/MAPK signaling pathway?

Neurofibromin, the product of the NF1 gene, acts as a GTPase-activating protein (GAP) for RAS, accelerating the conversion of active RAS-GTP to its inactive form RAS-GDP, thereby negatively regulating the RAS/MAPK pathway.

Are there any known interactions between NF1 and other tumor suppressor genes or oncogenes?

NF1 interacts with several proteins in cellular pathways, including c-Raf, mTOR, and adenylate cyclase. Its dysregulation can influence the activity of these proteins and associated pathways.

How does NF1 influence cell growth and differentiation?

By regulating the RAS/MAPK pathway, NF1 plays a crucial role in controlling cell growth, differentiation, and apoptosis.

What are the current therapeutic strategies targeting NF1-related disorders?

Current therapeutic strategies for NF1-related disorders include surgical removal of tumors, targeted therapies like MEK inhibitors for plexiform neurofibromas, and regular monitoring for associated complications.

What are the clinical manifestations of Neurofibromatosis Type 1, the disorder associated with NF1 mutations?

Neurofibromatosis Type 1 is characterized by multiple café-au-lait spots, skinfold freckling, neurofibromas, optic pathway gliomas, and certain bone abnormalities, among other manifestations.