| Cat. No. : |
Retn-69R |
| Description : |
Resistin, a product of the RSTN gene, is a peptide hormone belonging to the class of cysteine-rich secreted proteins (monomeric peptide contains 11 cysteine residues) referred to as the RELM family, and is also described as ADSF (Adipose Tissue-Specific Secretory Factor) or FIZZ3 (Found in Inflammatory Zone 3). Mouse resistin is expressed as a 114 amino acid prepeptide; its hydrofobic Nterminal 20 amino acid signal peptide is cleaved before its secretion. Mouse resistin circulates in blood as a homodimeric protein consisting of two 94 amino acid polypeptides, which are disulfide-linked via Cys26. Resistin may be an important link between obesity and insulin resistance. |
| Source : |
Escherichia Coli. |
| Specificity : |
The amino acid sequence of the Resistin Rat Recombinant is 100% homologous to the amino acid sequence of the Resistin Rat without signal sequence. |
| Purification Method : |
One -step procedure using affinity Ni-NTA chromatography. |
| Purity : |
>95% (SDS-PAGE analyzed). |
| Formulation : |
Sterile filtered and lyophilized from 0.5 mg/ml in 20mM Tris pH 8.0. |
| Solubility : |
Add 0.2 ml of deionized H2O and let the lyophilized pellet dissolve completely. |
| Applications : |
Western blotting. |
| Stability : |
Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C. The lyophilized protein remains stable until the expiry date when stored at -20°C. |
