Recombinant Rat Tgfa protein, His-tagged

• Cat.No.: Tgfa-523R
• Product Overview: Recombinant Rat Tgfa aa. (His42~Glu82) fused with N-terminal His tag was produced in E. coli cells.

Specification

• Description: Acts as an EGF receptor ligand; inhibits apoptosis that results from epithelial detachment from the extracellular matrix
• Source: E. coli
• Species: Rat
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: 12kDa as determined by SDS-PAGE reducing conditions
• Protein length: His42~Glu82
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: >92%
• Characteristic: The isoelectric point is 6.1.
• Applications: SDS-PAGE; WB; ELISA; IP; CoIP; Purification; Amine Reactive Labeling.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Concentration: 200μg/mL
• Storage buffer: PBS, pH7.4, containing 1mM DTT, 5% trehalose, 0.01% sarcosyl and Proclin300.
• Reconstitution: Reconstitute in PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex

Gene Information

• Gene Name: Tgfa transforming growth factor alpha [ Rattus norvegicus (Norway rat) ]
• Official Symbol: Tgfa
• Synonyms: Tgfa; transforming growth factor alpha; TGFAA; RATTGFAA; protransforming growth factor alpha
• Gene ID: 24827
• mRNA Refseq: NM_012671.2
• Protein Refseq: NP_036803.1
• UniProt ID: P01134