Active GMP Recombinant Human IL18 Protein

• Cat.No.: IL18-153HG
• Product Overview: Recombinant HumanIL18 protein was produced in HEK293 cell in an animal component free process under cGMP guidelines.

Specification

• Source: HEK293
• Species: Human
• Form: Lyophilized from a 0.2 m filtered solution of 20mM PBS, pH7.4, 6% mannitol.
• Bio-activity: 1.0*10^6IU
  Measured by its ability to induce IFN-gamma secretion by KG‐1 human acute myelogenous leukemia cells in the presence of TNF-alpha.
• Molecular Mass: The apparent molecular mass of recombinant Human IL18 is approximately 22 kDa in SDS-PAGE under reducing conditions.
• Protein length: Tyr37-Asp193
• AA Sequence: YFGKLESKLS VIRNLNDQVL FIDQGNRPLF EDMTDSDCRD NAPRTIFIISMYKDSQPRGM AVTISVKCEK ISTLSCENKI ISFKEMNPPD NIKDTKSDIIFFQRSVPGHD NKMQFESSSY EGYFLACEKE RDLFKLILKK EDELGDRSIMFTVQNEDVD
• Endotoxin: < 100 EU per mg of the protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE.
• Storage: Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
  It is not recommended to reconstitute to a concentration >100μg/ml.
  Dissolve the lyophilized protein in ddH2O.
  Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Gene Information

• Gene Name: IL18 interleukin 18 (interferon-gamma-inducing factor) [ Homo sapiens ]
• Official Symbol: IL18
• Synonyms: IL18; interleukin 18 (interferon-gamma-inducing factor); interleukin-18; IGIF; IL 1g; IL 18; IL1F4; IL-1 gamma; iboctadekin; interleukin-1 gamma; IFN-gamma-inducing factor; IL-18; IL-1g
• Gene ID: 3606
• mRNA Refseq: NM_001243211
• Protein Refseq: NP_001230140
• MIM: 600953
• UniProt ID: Q14116