Active GMP Recombinant Human IL3 protein

• Cat.No.: IL3-075HG
• Product Overview: Active GMP Recombinant Human IL3 protein was expressed in E. coli.

Specification

• Species: Human
• Source: E. coli
• Tag: Non
• Form: Lyophilized from a 0.2 µm filtered concentrated solution in PBS, 0.02% Tween-20, pH 7.0.
• Bio-activity: Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. The specific activity of recombinant human IL-3 is >1.0 x 106IU/mg, which is calibrated against the human IL-3 WHO International Standard (NIBSC code: 91/510).
• Molecular Mass: Approximately 15.0 kDa, a single non-glycosylated polypeptide chain containing 133 amino acids.
• AASequence: APMTQTTSLK TSWVNCSNMI DEIITHLKQP PLPLLDFNNL NGEDQDILME NNLRRPNLEA FNRAVKSLQN ASAIESILKN LLPCLPLATA APTRHPIHIK DGDWNEFRRK LTFYLKTLEN AQAQQTTLSL AIF
• Endotoxin: Less than 0.01 EU/µg of rHuIL-3 GMP as determined by LAL method.
• Purity: > 98 % by SDS-PAGE and HPLC analyses.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - 12 months from date of receipt, -20 to -70 °C as supplied. - 1 month, 2 to 8 °C under sterile conditions after reconstitution. - 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Gene Information

• Gene Name: IL3 interleukin 3 (colony-stimulating factor, multiple) [ Homo sapiens ]
• Official Symbol: IL3
• Synonyms: IL3; interleukin 3 (colony-stimulating factor, multiple); interleukin-3; hematopoietic growth factor; IL 3; mast cell growth factor; MCGF; MGC79398; MGC79399; MULTI CSF; multilineage colony stimulating factor; P cell stimulating factor; mast-cell growth factor; P-cell stimulating factor; P-cell-stimulating factor; multilineage-colony-stimulating factor; multipotential colony-stimulating factor; IL-3; MULTI-CSF
• Gene ID: 3562
• mRNA Refseq: NM_000588
• Protein Refseq: NP_000579
• MIM: 147740
• UniProt ID: P08700