Active Recombinant Cynomolgus IL25 protein, His&Avi-tagged, Biotinylated, Low Endotoxin

• Cat.No.: IL25-6358C
• Product Overview: Biotinylated Recombinant Cynomolgus IL25 protein(G7P9V9)(33-176 aa), fused with C-terminal His tag and N-terminal Avi tag, was expressed in HEK293.

Specification

• Species: Cynomolgus
• Source: HEK293
• Tag: Avi&His
• Protein Length: 33-176 aa
• Conjugation/Label: Biotin
• Form: Lyophilized from 0.22 μm filtered solution in PBS, 8% trehalose (pH 7.4).
• Bio-activity: Immobilized Human IL-17RB, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Cynomolgus IL-25, His Tag with the EC50 of 11.1ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 19.99 kDa. Due to glycosylation, the protein migrates to 25-32 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 0.01 EU per μg by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC
• Storage: Store at -20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water.